PMID- 10221657
OWN - NLM
STAT- MEDLINE
DCOM- 19990629
LR  - 20190513
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 11
IP  - 3
DP  - 1999 Mar
TI  - Partial block in B lymphocyte development at the transition into the pre-B cell
      receptor stage in Vpre-B1-deficient mice.
PG  - 453-60
AB  - The surrogate light chain (SL) is composed of two polypeptides, Vpre-B and
      lambda5. In large pre-BII cells the SL chain associates with Ig mu heavy chain
      (muH) to form the pre-B cell receptor (pre-BCR). In mice there are two Vpre-B
      genes which are 98% identical within the coding regions. The two genes are
      co-expressed at the RNA level and encode functional proteins that can assemble
      with lambda5. However, it is not known whether both gene products serve the same 
      function in vivo. Here we have established mice that lack the Vpre-B1 gene
      (VpreB1(-/-)), but still express the Vpre-B2 gene, both as RNA and protein. In
      Vpre-B1(-/-) mice, the bone marrow cellularity and the percentage of B220+ cells 
      is normal. However, among the B220+ cells, the percentage of pre-BI cells is
      increased, and the percentage of pre-BII and immature B cells is slightly
      decreased, suggesting that the lack of Vpre-B1 causes a partial block at the
      transition from pre-BI to pre-BII cells, i.e. into the pre-BCR stage. The number 
      of cells that produce a functional pre-BCR is thus lower, but the cells that
      reach this stage are normal as they can be expanded by proliferation and then
      differentiate into more mature cells. The spleens of Vpre-B1 homozygous mutant
      mice show normal numbers of B and T lymphocytes. Moreover, the Ig loci are
      allelicly excluded and the homozygous mutant mice respond with normal levels of
      antigen-specific antibodies to T-dependent antigens. These results demonstrate
      that VpreB2 alone is capable of supporting B lymphocyte development in the bone
      marrow and can give rise to immuno-competent cells in the periphery.
FAU - Martensson, A
AU  - Martensson A
AD  - Department of Cell and Molecular Biology, University of Lund, Sweden.
FAU - Argon, Y
AU  - Argon Y
FAU - Melchers, F
AU  - Melchers F
FAU - Dul, J L
AU  - Dul JL
FAU - Martensson, I L
AU  - Martensson IL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Immunoglobulin Light Chains)
RN  - 0 (Immunoglobulin Light Chains, Surrogate)
RN  - 0 (Immunoglobulin mu-Chains)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Antigen)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/*immunology
MH  - Bone Marrow Cells/immunology
MH  - Hematopoiesis/*genetics
MH  - Hematopoietic Stem Cells/*immunology
MH  - Immunoglobulin Light Chains
MH  - Immunoglobulin Light Chains, Surrogate
MH  - Immunoglobulin mu-Chains/immunology
MH  - Membrane Glycoproteins/*genetics/immunology
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Receptors, Antigen/*genetics/immunology
MH  - Spleen/immunology
EDAT- 1999/04/30 00:00
MHDA- 1999/04/30 00:01
CRDT- 1999/04/30 00:00
PHST- 1999/04/30 00:00 [pubmed]
PHST- 1999/04/30 00:01 [medline]
PHST- 1999/04/30 00:00 [entrez]
AID - 10.1093/intimm/11.3.453 [doi]
PST - ppublish
SO  - Int Immunol. 1999 Mar;11(3):453-60. doi: 10.1093/intimm/11.3.453.