PMID- 10221542
OWN - NLM
STAT- MEDLINE
DCOM- 19990728
LR  - 20190905
IS  - 8756-3282 (Print)
IS  - 1873-2763 (Linking)
VI  - 24
IP  - 4
DP  - 1999 Apr
TI  - Identification of a novel parathyroid hormone-responsive gene in human
      osteoblastic cells.
PG  - 305-13
AB  - Parathyroid hormone (PTH) is a potent stimulator of osteoblastic cell function in
      vitro and bone resorption and formation in vivo; however, the details of the
      molecular mechanism(s) responsible for PTH action and the regulation of gene
      expression in response to PTH remain unknown. In this study, we employed an mRNA 
      differential display (DRD) approach to examine the initial events in gene
      expression in human osteoblast-like SaoS-2/B10 cells exposed to 10(-7) mol/L
      bPTH(1-34). This approach identified several differentially regulated mRNA
      species, including a novel paired-class homeobox protein, osteoblast-specific
      factor-2 (OSF-2), and a unique clone with no known sequence homology (clone G18).
      G18 is a previously unidentified human gene, expressed in a wide variety of human
      tissues, including heart, brain, placenta, skeletal muscle, and kidney, and is
      regulated by PTH in osteoblastic cells in vitro. This mRNA appears to be the
      product of a single gene, which is alternatively spliced to produce multiple
      transcript sizes observed in several tissues, except bone and bone-derived cells,
      in which a single predominant approximately 1.8 kb transcript is observed. Our
      study has identified several genes that have expression altered significantly by 
      treatment with bPTH(1-34), and which may provide insight into the immediate
      effects of PTH on osteoblast-like cells and ultimately on the mechanism of action
      and bioactivity of PTH.
FAU - Adams, A E
AU  - Adams AE
AD  - Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Institute
      of Medicine and Harvard Medical School, Boston, MA 02215, USA.
      aadams@bidmc.harvard.edu
FAU - Rosenblatt, M
AU  - Rosenblatt M
FAU - Suva, L J
AU  - Suva LJ
LA  - eng
SI  - GENBANK/AF095769
SI  - GENBANK/AF095770
SI  - GENBANK/AF095771
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Bone
JT  - Bone
JID - 8504048
RN  - 0 (Parathyroid Hormone)
RN  - 0 (Peptide Fragments)
RN  - 12583-68-5 (parathyroid hormone (1-34), bovine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Gene Expression Regulation/*physiology
MH  - *Genes, Immediate-Early
MH  - Humans
MH  - Molecular Sequence Data
MH  - Osteoblasts/*physiology
MH  - Parathyroid Hormone/*genetics/*physiology
MH  - Peptide Fragments/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 1999/04/30 00:00
MHDA- 1999/04/30 00:01
CRDT- 1999/04/30 00:00
PHST- 1999/04/30 00:00 [pubmed]
PHST- 1999/04/30 00:01 [medline]
PHST- 1999/04/30 00:00 [entrez]
AID - S8756-3282(98)00188-4 [pii]
AID - 10.1016/s8756-3282(98)00188-4 [doi]
PST - ppublish
SO  - Bone. 1999 Apr;24(4):305-13. doi: 10.1016/s8756-3282(98)00188-4.