PMID- 10220587
OWN - NLM
STAT- MEDLINE
DCOM- 19990811
LR  - 20190513
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 125
IP  - 5
DP  - 1999 May
TI  - Molecular cloning of mouse p47, a second group mammalian RuvB DNA helicase-like
      protein: homology with those from human and Saccharomyces cerevisiae.
PG  - 939-46
AB  - A 47k protein (p47) in a high-salt buffer extract of a rat liver nuclear matrix
      fraction was purified by means of a wheat germ agglutinin affinity column,
      reversed phase HPLC, and SDS-PAGE, and partial amino acid sequences were
      analyzed. Based on these sequences, the mouse cDNA of the protein was cloned and 
      sequenced, and its amino acid sequence was deduced. Mouse p47 consists of 463
      amino acid residues with a molecular weight of 51,112. The amino acid sequences
      of human and Saccharomyces cerevisiae p47s were also deduced from the nucleotide 
      sequences of "expressed sequence tag" fragments and genomic DNA, respectively.
      These sequences contain helicase motifs and show homology to bacterial RuvB DNA
      helicases acting in homologous recombination. They also show homology with the
      putative mammalian helicases p50/TIP49 and RUVBL1. Comparison of the amino acid
      sequences of p47 group proteins and those of p50/TIP49 group proteins revealed
      the p47 group proteins to comprise a group distinct from the p50/TIP49 proteins. 
      Ultracentrifugation and gel filtration analyses showed that p47 in the rat liver 
      cytosol fraction exists as large complexes of 697k.
FAU - Gohshi, T
AU  - Gohshi T
AD  - Department of Biochemistry, Faculty of Science, Okayama University Dental School,
      Okayama, 700-8525, Japan.
FAU - Shimada, M
AU  - Shimada M
FAU - Kawahire, S
AU  - Kawahire S
FAU - Imai, N
AU  - Imai N
FAU - Ichimura, T
AU  - Ichimura T
FAU - Omata, S
AU  - Omata S
FAU - Horigome, T
AU  - Horigome T
LA  - eng
SI  - GENBANK/AB013912
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (RuvB protein, Bacteria)
RN  - EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (RUVBL2 protein, mouse)
SB  - IM
MH  - ATPases Associated with Diverse Cellular Activities
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacterial Proteins/chemistry/*genetics
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA Helicases/chemistry/*genetics
MH  - DNA, Complementary
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Rats
MH  - Saccharomyces cerevisiae/*genetics
MH  - Sequence Homology, Amino Acid
EDAT- 1999/04/30 00:00
MHDA- 1999/04/30 00:01
CRDT- 1999/04/30 00:00
PHST- 1999/04/30 00:00 [pubmed]
PHST- 1999/04/30 00:01 [medline]
PHST- 1999/04/30 00:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a022372 [doi]
PST - ppublish
SO  - J Biochem. 1999 May;125(5):939-46. doi: 10.1093/oxfordjournals.jbchem.a022372.