PMID- 10220415
OWN - NLM
STAT- MEDLINE
DCOM- 19990610
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 9
DP  - 1999 Apr 27
TI  - Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants
      lacking the KIF3A subunit of kinesin-II.
PG  - 5043-8
AB  - The embryonic cellular events that set the asymmetry of the genetic control
      circuit controlling left-right (L-R) axis determination in mammals are poorly
      understood. New insight into this problem was obtained by analyzing mouse mutants
      lacking the KIF3A motor subunit of the kinesin-II motor complex. Embryos lacking 
      KIF3A die at 10 days postcoitum, exhibit randomized establishment of L-R
      asymmetry, and display numerous structural abnormalities. The earliest detectable
      abnormality in KIF3A mutant embryos is found at day 7.5, where scanning electron 
      microscopy reveals loss of cilia ordinarily present on cells of the wild-type
      embryonic node, which is thought to play an important role in setting the initial
      L-R asymmetry. This cellular phenotype is observed before the earliest reported
      time of asymmetric expression of markers of the L-R signaling pathway. These
      observations demonstrate that the kinesin-based transport pathway needed for
      flagellar and ciliary morphogenesis is conserved from Chlamydomonas to mammals
      and support the view that embryonic cilia play a role in the earliest cellular
      determinative events establishing L-R asymmetry.
FAU - Marszalek, J R
AU  - Marszalek JR
AD  - Division of Cellular and Molecular Medicine, University of California, San Diego 
      School of Medicine, La Jolla, CA, USA.
FAU - Ruiz-Lozano, P
AU  - Ruiz-Lozano P
FAU - Roberts, E
AU  - Roberts E
FAU - Chien, K R
AU  - Chien KR
FAU - Goldstein, L S
AU  - Goldstein LS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Muscle Proteins)
RN  - 147096-75-1 (kinesin-II)
RN  - EC 3.6.4.4 (Kinesin)
SB  - IM
MH  - Animals
MH  - Calcium-Binding Proteins/*genetics
MH  - Cilia/*genetics/ultrastructure
MH  - Embryonic and Fetal Development/*genetics
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Kinesin
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Morphogenesis/genetics
MH  - Muscle Proteins/*genetics
MH  - Mutation
MH  - Pregnancy
PMC - PMC21813
EDAT- 1999/04/29 00:00
MHDA- 1999/04/29 00:01
CRDT- 1999/04/29 00:00
PHST- 1999/04/29 00:00 [pubmed]
PHST- 1999/04/29 00:01 [medline]
PHST- 1999/04/29 00:00 [entrez]
AID - 10.1073/pnas.96.9.5043 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5043-8. doi: 10.1073/pnas.96.9.5043.