PMID- 10220412
OWN - NLM
STAT- MEDLINE
DCOM- 19990610
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 9
DP  - 1999 Apr 27
TI  - Induction of a secreted protein by the myxoid liposarcoma oncogene.
PG  - 5025-30
AB  - The TLS-CHOP oncoprotein, found in the majority of human myxoid liposarcomas,
      consists of a fusion between the transcription factor CHOP/GADD153 and the N
      terminus of an RNA-binding protein TLS/FUS. Clinical correlation and in vitro
      transformation assays indicate that the N terminus of TLS plays an important role
      in oncogenesis by TLS-CHOP. Until now, however, the only activity attributed to
      the oncoprotein is that of inhibiting the binding of transcription factors of the
      C/EBP class to certain adipogenic target genes, a function that TLS-CHOP shares
      with the nononcogenic CHOP protein. Here we report the isolation of a gene,
      DOL54, that is activated in primary fibroblasts by the expression of TLS-CHOP.
      DOL54 is expressed in the neoplastic component of human myxoid liposarcomas and
      increases the tumorigenicity of cells injected in nude mice. Activation of DOL54 
      requires an intact DNA-binding and dimerization domain in TLS-CHOP, a suitable
      cellular dimerization partner, and depends on the TLS N terminus. Normal
      adipocytic differentiation is associated with an early and transient expression
      of DOL54, and the gene encodes a secreted protein that is tightly associated with
      the cell surface or extracellular matrix. TLS-CHOP thus leads to the unscheduled 
      expression of a gene that is normally associated with adipocytic differentiation.
FAU - Kuroda, M
AU  - Kuroda M
AD  - Skirball Institute of Biomolecular Medicine, Departments of Medicine, Cell
      Biology, and the Kaplan Cancer Center, New York University Medical Center, New
      York, NY 10016, USA.
FAU - Wang, X
AU  - Wang X
FAU - Sok, J
AU  - Sok J
FAU - Yin, Y
AU  - Yin Y
FAU - Chung, P
AU  - Chung P
FAU - Giannotti, J W
AU  - Giannotti JW
FAU - Jacobs, K A
AU  - Jacobs KA
FAU - Fitz, L J
AU  - Fitz LJ
FAU - Murtha-Riel, P
AU  - Murtha-Riel P
FAU - Turner, K J
AU  - Turner KJ
FAU - Ron, D
AU  - Ron D
LA  - eng
SI  - GENBANK/U70316
GR  - CA60945/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (RNA-Binding Protein FUS)
RN  - 0 (TLS-CHOP fusion protein, human)
RN  - 147336-12-7 (Transcription Factor CHOP)
SB  - IM
MH  - Animals
MH  - *CCAAT-Enhancer-Binding Proteins
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Fibroblasts/metabolism
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Liposarcoma, Myxoid/*genetics/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*genetics/metabolism
MH  - Nuclear Proteins/*genetics
MH  - Oncogene Proteins, Fusion/*genetics
MH  - *RNA-Binding Protein FUS
MH  - Transcription Factor CHOP
PMC - PMC21810
EDAT- 1999/04/29 00:00
MHDA- 1999/04/29 00:01
CRDT- 1999/04/29 00:00
PHST- 1999/04/29 00:00 [pubmed]
PHST- 1999/04/29 00:01 [medline]
PHST- 1999/04/29 00:00 [entrez]
AID - 10.1073/pnas.96.9.5025 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5025-30. doi: 10.1073/pnas.96.9.5025.