PMID- 10219247
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 97
IP  - 2
DP  - 1999 Apr 16
TI  - Crystal structure of the interleukin-4/receptor alpha chain complex reveals a
      mosaic binding interface.
PG  - 271-81
AB  - Interleukin-4 (IL-4) is a principal regulatory cytokine during an immune response
      and a crucial determinant for allergy and asthma. IL-4 binds with high affinity
      and specificity to the ectodomain of the IL-4 receptor alpha chain (IL4-BP).
      Subsequently, this intermediate complex recruits the common gamma chain (gamma
      c), thereby initiating transmembrane signaling. The crystal structure of the
      intermediate complex between human IL-4 and IL4-BP was determined at 2.3 A
      resolution. It reveals a novel spatial orientation of the two proteins, a small
      but unexpected conformational change in the receptor-bound IL-4, and an interface
      with three separate clusters of trans-interacting residues. Novel insights on
      ligand binding in the cytokine receptor family and a paradigm for receptors of
      IL-2, IL-7, IL-9, and IL-15, which all utilize gamma c, are provided.
FAU - Hage, T
AU  - Hage T
AD  - Institut fur Physiologische Chemie II, Theodor-Boveri-Institut fur Biowissen
      Schaften (Biozentrum), Universitat Wurzburg, Germany.
FAU - Sebald, W
AU  - Sebald W
FAU - Reinemer, P
AU  - Reinemer P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Receptors, Interleukin-4)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Crystallography, X-Ray
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-4/*chemistry/metabolism
MH  - Macromolecular Substances
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Conformation
MH  - Receptors, Cytokine/chemistry/genetics
MH  - Receptors, Interleukin-4/*chemistry/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
EDAT- 1999/04/29 00:00
MHDA- 1999/04/29 00:01
CRDT- 1999/04/29 00:00
PHST- 1999/04/29 00:00 [pubmed]
PHST- 1999/04/29 00:01 [medline]
PHST- 1999/04/29 00:00 [entrez]
AID - S0092-8674(00)80736-9 [pii]
AID - 10.1016/s0092-8674(00)80736-9 [doi]
PST - ppublish
SO  - Cell. 1999 Apr 16;97(2):271-81. doi: 10.1016/s0092-8674(00)80736-9.