PMID- 10219243
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 97
IP  - 2
DP  - 1999 Apr 16
TI  - The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent
      signals to actin assembly.
PG  - 221-31
AB  - Although small GTP-binding proteins of the Rho family have been implicated in
      signaling to the actin cytoskeleton, the exact nature of the linkage has remained
      obscure. We describe a novel mechanism that links one Rho family member, Cdc42,
      to actin polymerization. N-WASP, a ubiquitously expressed Cdc42-interacting
      protein, is required for Cdc42-stimulated actin polymerization in Xenopus egg
      extracts. The C terminus of N-WASP binds to the Arp2/3 complex and dramatically
      stimulates its ability to nucleate actin polymerization. Although full-length
      N-WASP is less effective, its activity can be greatly enhanced by Cdc42 and
      phosphatidylinositol (4,5) bisphosphate. Therefore, N-WASP and the Arp2/3 complex
      comprise a core mechanism that directly connects signal transduction pathways to 
      the stimulation of actin polymerization.
FAU - Rohatgi, R
AU  - Rohatgi R
AD  - Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, 
      USA.
FAU - Ma, L
AU  - Ma L
FAU - Miki, H
AU  - Miki H
FAU - Lopez, M
AU  - Lopez M
FAU - Kirchhausen, T
AU  - Kirchhausen T
FAU - Takenawa, T
AU  - Takenawa T
FAU - Kirschner, M W
AU  - Kirschner MW
LA  - eng
GR  - 5PO1 HL 59561-02/HL/NHLBI NIH HHS/United States
GR  - GM26875/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Actin-Related Protein 2)
RN  - 0 (Actin-Related Protein 3)
RN  - 0 (Actins)
RN  - 0 (Biopolymers)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (N-WASP protein, rat)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Phosphatidylinositol 4,5-Diphosphate)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Wiskott-Aldrich Syndrome Protein, Neuronal)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein)
SB  - IM
MH  - Actin-Related Protein 2
MH  - Actin-Related Protein 3
MH  - Actins/*metabolism
MH  - Animals
MH  - Biopolymers/metabolism
MH  - Cattle
MH  - Cell Cycle Proteins/*metabolism
MH  - *Cytoskeletal Proteins
MH  - Female
MH  - GTP-Binding Proteins/*metabolism
MH  - In Vitro Techniques
MH  - Macromolecular Substances
MH  - Models, Biological
MH  - Nerve Tissue Proteins/chemistry/genetics/*metabolism
MH  - Oocytes/metabolism
MH  - Peptide Fragments/chemistry/genetics/metabolism
MH  - Phosphatidylinositol 4,5-Diphosphate/metabolism
MH  - Rats
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Signal Transduction
MH  - Wiskott-Aldrich Syndrome Protein, Neuronal
MH  - Xenopus
MH  - cdc42 GTP-Binding Protein
EDAT- 1999/04/29 00:00
MHDA- 1999/04/29 00:01
CRDT- 1999/04/29 00:00
PHST- 1999/04/29 00:00 [pubmed]
PHST- 1999/04/29 00:01 [medline]
PHST- 1999/04/29 00:00 [entrez]
AID - S0092-8674(00)80732-1 [pii]
AID - 10.1016/s0092-8674(00)80732-1 [doi]
PST - ppublish
SO  - Cell. 1999 Apr 16;97(2):221-31. doi: 10.1016/s0092-8674(00)80732-1.