PMID- 10219089
OWN - NLM
STAT- MEDLINE
DCOM- 19990624
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 10
DP  - 1999 May 15
TI  - Isolation of Ku70-binding proteins (KUBs).
PG  - 2165-74
AB  - DNA-dependent protein kinase (DNA-PK) plays a critical role in resealing DNA
      double-stand breaks by non-homologous end joining. Aside from DNA-PK, XRCC4 and
      DNA ligase IV, other proteins which play a role(s) in this repair pathway remain 
      unknown; DNA-PK contains a catalytic subunit (DNA-PKcs) and a DNA binding subunit
      (Ku70 and Ku80). We isolated Ku70-binding proteins (KUB1-KUB4) using yeast
      two-hybrid analyses. Sequence analyses revealed KUB1 to be apolipoprotein J
      (apoJ), also known as X-ray-inducible transcript 8 (XIP8), testosterone-repressed
      prostate message-2 (TRPM-2) and clusterin. KUB2 is Ku80. KUB3 and KUB4 are
      unknown, >10 kb trans-cripts. Interactions of apoJ/XIP8 or KUB3 with Ku70 were
      confirmed by co-immunoprecipitation analyses in MCF-7:WS8 breast cancer or IMR-90
      normal lung fibroblast cells, respectively. The interaction of apoJ/XIP8 with
      Ku70 was confirmed by far-western analyses. Stable over-expression of full-length
      apoJ/XIP8 in MCF-7:WS8 caused decreased Ku70/Ku80 DNA end binding that was
      restored by apoJ/XIP8 monoclonal antibodies. The role of apoJ/XIP8 in ionizing
      radiation resistance/sensitivity is under investigation.
FAU - Yang, C R
AU  - Yang CR
AD  - Department of Radiation Oncology and Department of Pharmacology and the Ireland
      Cancer Center,Laboratory of Molecular Stress Responses, Case Western Reserve
      University, BRB-326 East,10900 Euclid Avenue, Cleveland, OH 44106-4942, USA.
FAU - Yeh, S
AU  - Yeh S
FAU - Leskov, K
AU  - Leskov K
FAU - Odegaard, E
AU  - Odegaard E
FAU - Hsu, H L
AU  - Hsu HL
FAU - Chang, C
AU  - Chang C
FAU - Kinsella, T J
AU  - Kinsella TJ
FAU - Chen, D J
AU  - Chen DJ
FAU - Boothman, D A
AU  - Boothman DA
LA  - eng
GR  - R01 CA139217/CA/NCI NIH HHS/United States
GR  - CA-50595/CA/NCI NIH HHS/United States
GR  - CA-ES78530/CA/NCI NIH HHS/United States
GR  - CA50519/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Antigens, Nuclear)
RN  - 0 (CLU protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Clu protein, mouse)
RN  - 0 (Clusterin)
RN  - 0 (DNA Probes)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (KUB3 protein, human)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (high affinity DNA-binding factor, S cerevisiae)
RN  - EC 2.7.11.1 (DNA-Activated Protein Kinase)
RN  - EC 2.7.11.1 (PRKDC protein, human)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (XRCC5 protein, human)
RN  - EC 3.6.4.12 (Xrcc6 protein, human)
RN  - EC 3.6.4.12 (Xrcc6 protein, mouse)
RN  - EC 4.2.99.- (Ku Autoantigen)
SB  - IM
MH  - Animals
MH  - *Antigens, Nuclear
MH  - Base Sequence
MH  - Carrier Proteins/genetics/*isolation & purification/*metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Clusterin
MH  - *DNA Helicases
MH  - DNA Probes/genetics
MH  - DNA Repair
MH  - DNA-Activated Protein Kinase
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Glycoproteins/metabolism
MH  - Humans
MH  - Ku Autoantigen
MH  - Mice
MH  - *Molecular Chaperones
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Protein Binding
MH  - Protein-Serine-Threonine Kinases/metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - *Saccharomyces cerevisiae Proteins
MH  - Tumor Cells, Cultured
PMC - PMC148436
EDAT- 1999/04/29 00:00
MHDA- 1999/04/29 00:01
CRDT- 1999/04/29 00:00
PHST- 1999/04/29 00:00 [pubmed]
PHST- 1999/04/29 00:01 [medline]
PHST- 1999/04/29 00:00 [entrez]
AID - gkc350 [pii]
AID - 10.1093/nar/27.10.2165 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 May 15;27(10):2165-74. doi: 10.1093/nar/27.10.2165.