PMID- 10218588
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 447
IP  - 1
DP  - 1999 Mar 19
TI  - The cleavage site specificity of human prostate specific antigen for insulin-like
      growth factor binding protein-3.
PG  - 87-90
AB  - The cleavage site of human insulin-like growth factor binding protein-3 by
      urinary prostate specific antigen was examined. Human insulin-like growth factor 
      binding protein-3 was incubated with urinary prostate specific antigen at 37
      degrees C and its proteolyzed fragments were separated by a reversed phase HPLC
      followed by N-terminal amino acid sequence analysis, demonstrating that the
      cleavage mainly occurred at Tyr-159. The synthetic peptide including Tyr-159 was 
      also cleaved at the same site, although its reaction rate was relatively low.
      These results indicate that human insulin-like growth factor binding protein-3 is
      specifically cleaved at Tyr-159 by prostate specific antigen. Human insulin-like 
      growth factor binding protein-3 was previously reported to be cleaved at five
      sites including Arg-97, Arg-132, Tyr-159, Phe-173 and Arg-179 by another group,
      however, prostate specific antigen preparation is possibly contaminated by
      trypsin-like protease. In contrast, our purified urinary prostate specific
      antigen had only a chymotrypsin-like activity, demonstrating that prostate
      specific antigen has the high substrate specificity for human insulin-like growth
      factor binding protein-3.
FAU - Okabe, E
AU  - Okabe E
AD  - Development and Research Laboratories, JCR Pharmaceuticals, Kobe, Japan.
      kajihara@jcrpharm.co.jp
FAU - Kajihara, J
AU  - Kajihara J
FAU - Usami, Y
AU  - Usami Y
FAU - Hirano, K
AU  - Hirano K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Insulin-Like Growth Factor Binding Protein 3)
RN  - EC 3.4.21.1 (Chymotrypsin)
RN  - EC 3.4.21.77 (Prostate-Specific Antigen)
SB  - IM
MH  - Amino Acid Sequence
MH  - Chymotrypsin/*metabolism
MH  - Humans
MH  - Insulin-Like Growth Factor Binding Protein 3/*metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - Prostate-Specific Antigen/*isolation & purification/*metabolism/urine
MH  - Semen/enzymology
MH  - Substrate Specificity
EDAT- 1999/04/28 00:00
MHDA- 1999/04/28 00:01
CRDT- 1999/04/28 00:00
PHST- 1999/04/28 00:00 [pubmed]
PHST- 1999/04/28 00:01 [medline]
PHST- 1999/04/28 00:00 [entrez]
AID - S0014579399002756 [pii]
AID - 10.1016/s0014-5793(99)00275-6 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Mar 19;447(1):87-90. doi: 10.1016/s0014-5793(99)00275-6.