PMID- 10218578
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 447
IP  - 1
DP  - 1999 Mar 19
TI  - Demonstration of the pathogenic effect of point mutated keratin 9 in vivo.
PG  - 39-43
AB  - A wild type keratin 9 (K9) cDNA and a point mutated keratin 9 cDNA were injected 
      subcutaneously into mouse skin. The hemagglutinin tag staining of the wild type
      K9 cDNA injected specimens mainly showed a homogeneous pattern, whereas the point
      mutated K9 cDNA injected specimens mainly showed a granular pattern in the
      suprabasal cells. Double staining of K9 and the endogenous keratin revealed the
      incorporation of de novo synthesized K9 into the keratin network. These results
      demonstrate that (1) a naked DNA transfection into mouse skin can detect the
      pathogenic changes of point mutated keratin in vivo and (2) the keratin 9
      mutation disrupts the keratin network formation in the suprabasal cells in vivo.
FAU - Kobayashi, S
AU  - Kobayashi S
AD  - Department of Dermatology, Graduate School of Medicine, Kyoto University, Japan.
FAU - Kore-eda, S
AU  - Kore-eda S
FAU - Tanaka, T
AU  - Tanaka T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Recombinant)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Animals
MH  - DNA, Recombinant/pharmacology
MH  - Humans
MH  - Injections, Subcutaneous
MH  - Intermediate Filaments/*pathology
MH  - Keratins/*genetics/*toxicity
MH  - Keratoderma, Palmoplantar/etiology/genetics
MH  - Mice
MH  - *Point Mutation
MH  - *Toxicity Tests
MH  - Transfection
EDAT- 1999/04/28 00:00
MHDA- 1999/04/28 00:01
CRDT- 1999/04/28 00:00
PHST- 1999/04/28 00:00 [pubmed]
PHST- 1999/04/28 00:01 [medline]
PHST- 1999/04/28 00:00 [entrez]
AID - S0014-5793(99)00233-1 [pii]
AID - 10.1016/s0014-5793(99)00233-1 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Mar 19;447(1):39-43. doi: 10.1016/s0014-5793(99)00233-1.