PMID- 10217420
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 448
IP  - 1
DP  - 1999 Apr 1
TI  - The RAMP2/CRLR complex is a functional adrenomedullin receptor in human
      endothelial and vascular smooth muscle cells.
PG  - 111-4
AB  - Adrenomedullin, a potently hypotensive peptide isolated from human
      pheochromocytoma, is known to elicit a rise in cAMP levels within mammalian
      endothelial and smooth muscle cells. Until now, however, little has been known
      about the adrenomedullin receptor. Recently, a group called receptor
      activity-modifying proteins that complex with the calcitonin receptor-like
      receptor, and thereby regulate its transport and ligand specificity, were
      identified. Here we show that mRNA for both the calcitonin receptor-like receptor
      and the receptor activity-modifying protein 2, but not the receptor
      activity-modifying protein 1 or receptor activity-modifying protein 3, are
      expressed in human endothelial and vascular smooth muscle cells. We also found
      that adrenomedullin increased cAMP levels in HeLa EBNA and 293 EBNA cells,
      expressing both the receptor activity-modifying protein 2 and calcitonin
      receptor-like receptor proteins. Thus, the receptor activity-modifying protein
      2/calcitonin receptor-like receptor complex apparently serves as a functional
      adrenomedullin receptor in human endothelial and vascular smooth muscle cells.
FAU - Kamitani, S
AU  - Kamitani S
AD  - Shionogi Institute for Medical Science, Shionogi and Co. Ltd., Osaka, Japan.
FAU - Asakawa, M
AU  - Asakawa M
FAU - Shimekake, Y
AU  - Shimekake Y
FAU - Kuwasako, K
AU  - Kuwasako K
FAU - Nakahara, K
AU  - Nakahara K
FAU - Sakata, T
AU  - Sakata T
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (CALCRL protein, human)
RN  - 0 (Calcitonin Receptor-Like Protein)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptides)
RN  - 0 (RAMP2 protein, human)
RN  - 0 (Receptor Activity-Modifying Protein 2)
RN  - 0 (Receptor Activity-Modifying Proteins)
RN  - 0 (Receptors, Adrenomedullin)
RN  - 0 (Receptors, Calcitonin)
RN  - 0 (Receptors, Peptide)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 148498-78-6 (Adrenomedullin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Adrenomedullin
MH  - Calcitonin Gene-Related Peptide/*metabolism/pharmacology
MH  - Calcitonin Receptor-Like Protein
MH  - Cells, Cultured
MH  - Cyclic AMP/metabolism
MH  - Endothelium, Vascular/cytology/metabolism
MH  - Gene Expression
MH  - HeLa Cells
MH  - Humans
MH  - Intracellular Fluid/metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Membrane Proteins/genetics/*metabolism
MH  - Muscle, Smooth, Vascular/cytology/metabolism
MH  - Peptides/*metabolism/pharmacology
MH  - Receptor Activity-Modifying Protein 2
MH  - Receptor Activity-Modifying Proteins
MH  - Receptors, Adrenomedullin
MH  - Receptors, Calcitonin/genetics/*metabolism
MH  - *Receptors, Peptide
MH  - Recombinant Fusion Proteins/genetics/metabolism
EDAT- 1999/04/27 00:00
MHDA- 1999/04/27 00:01
CRDT- 1999/04/27 00:00
PHST- 1999/04/27 00:00 [pubmed]
PHST- 1999/04/27 00:01 [medline]
PHST- 1999/04/27 00:00 [entrez]
AID - S0014-5793(99)00358-0 [pii]
AID - 10.1016/s0014-5793(99)00358-0 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Apr 1;448(1):111-4. doi: 10.1016/s0014-5793(99)00358-0.