PMID- 10217254
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 72
IP  - 5
DP  - 1999 May
TI  - Isolation of 10 differentially expressed cDNAs in differentiated Neuro2a cells
      induced through controlled expression of the GD3 synthase gene.
PG  - 1781-90
AB  - Recently, we showed that transfection of GD3 synthase cDNA into Neuro2a cells, a 
      mouse neuroblastoma cell line, causes cell differentiation with neurite
      sprouting. In a search for the genes involved in this ganglioside-induced Neuro2a
      differentiation, we used a tetracycline-regulated GD3 synthase cDNA expression
      system combined with differential display PCRs to identify mRNAs that were
      differentially expressed at four representative time points during the process.
      We report here the identification of 10 mRNAs that are expressed highly at the
      Neuro2a differentiated stage. These cDNAs were named GDAP1-GDAP10 for
      (ganglioside-induced differentiation-associated protein) cDNAs. It is interesting
      that in retinoic acid-induced neural differentiated mouse embryonic carcinoma P19
      cells, GDAP mRNA expression levels were also up-regulated (except that of GDAP3),
      ranging from three to >10 times compared with nondifferentiated P19 cells. All
      the GDAP genes (except that of GDAP3) were developmentally regulated. The GDAP1, 
      2, 6, 8, and 10 mRNAs were expressed highly in the adult mouse brain, whereas all
      the other GDAP mRNAs were expressed in most tissues. Our results suggested that
      these GDAP genes might be involved in the signal transduction pathway that is
      triggered through the expression of a single sialyltransferase gene to induce
      neurite-like differentiation of Neuro2a cells.
FAU - Liu, H
AU  - Liu H
AD  - Department of Molecular Glycobiology, Institute of Physical and Chemical
      Research, Wako, Saitama, Japan.
FAU - Nakagawa, T
AU  - Nakagawa T
FAU - Kanematsu, T
AU  - Kanematsu T
FAU - Uchida, T
AU  - Uchida T
FAU - Tsuji, S
AU  - Tsuji S
LA  - eng
SI  - GENBANK/Y17849
SI  - GENBANK/Y17850
SI  - GENBANK/Y17851
SI  - GENBANK/Y17852
SI  - GENBANK/Y17853
SI  - GENBANK/Y17854
SI  - GENBANK/Y17855
SI  - GENBANK/Y17856
SI  - GENBANK/Y17857
SI  - GENBANK/Y17858
SI  - GENBANK/Y17859
SI  - GENBANK/Y17860
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (DNA, Complementary)
RN  - 0 (GDAP protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 5688UTC01R (Tretinoin)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - EC 2.4.99.8 (alpha-N-acetylneuraminate alpha-2,8-sialyltransferase)
SB  - IM
MH  - Aging/metabolism
MH  - Amino Acid Sequence/genetics
MH  - Animals
MH  - Brain/growth & development/metabolism
MH  - Cell Differentiation/physiology
MH  - DNA, Complementary/*isolation & purification
MH  - Gene Expression Regulation/*physiology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*genetics
MH  - Sialyltransferases/*genetics
MH  - Tretinoin/pharmacology
MH  - Tumor Cells, Cultured/drug effects/metabolism/pathology
EDAT- 1999/04/27 00:00
MHDA- 1999/04/27 00:01
CRDT- 1999/04/27 00:00
PHST- 1999/04/27 00:00 [pubmed]
PHST- 1999/04/27 00:01 [medline]
PHST- 1999/04/27 00:00 [entrez]
AID - 10.1046/j.1471-4159.1999.0721781.x [doi]
PST - ppublish
SO  - J Neurochem. 1999 May;72(5):1781-90. doi: 10.1046/j.1471-4159.1999.0721781.x.