PMID- 10216252
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 230
IP  - 2
DP  - 1999 Apr 16
TI  - Isolation, sequencing and expression of RED, a novel human gene encoding an
      acidic-basic dipeptide repeat.
PG  - 145-54
AB  - A novel human gene RED, and the murine homologue, MuRED, were cloned. These genes
      were named after the extensive stretch of alternating arginine (R) and glutamic
      acid (E) or aspartic acid (D) residues that they contain. We term this the 'RED' 
      repeat. The genes of both species were expressed in a wide range of tissues and
      we have mapped the human gene to chromosome 5q22-24. MuRED and RED shared 98%
      sequence identity at the amino acid level. The open reading frame of both genes
      encodes a 557 amino acid protein. RED fused to a fluorescent tag was expressed in
      nuclei of transfected cells and localised to nuclear dots. Co-localisation
      studies showed that these nuclear dots did not contain either PML or Coilin,
      which are commonly found in the POD or coiled body nuclear compartments. Deletion
      of the amino terminal 265 amino acids resulted in a failure to sort efficiently
      to the nucleus, though nuclear dots were formed. Deletion of a further 50 amino
      acids from the amino terminus generates a protein that can sort to the nucleus
      but is unable to generate nuclear dots. Neither construct localised to the
      nucleolus. The characteristics of RED and its nuclear localisation implicate it
      as a regulatory protein, possibly involved in transcription.
FAU - Assier, E
AU  - Assier E
AD  - INSERM U396, Institut Biomedical des Cordeliers, 15 rue de l'Ecole de Medecine,
      75006, Paris, France.
FAU - Bouzinba-Segard, H
AU  - Bouzinba-Segard H
FAU - Stolzenberg, M C
AU  - Stolzenberg MC
FAU - Stephens, R
AU  - Stephens R
FAU - Bardos, J
AU  - Bardos J
FAU - Freemont, P
AU  - Freemont P
FAU - Charron, D
AU  - Charron D
FAU - Trowsdale, J
AU  - Trowsdale J
FAU - Rich, T
AU  - Rich T
LA  - eng
SI  - GENBANK/AJ005579
SI  - GENBANK/AJ006130
GR  - Wellcome Trust/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Cytokines)
RN  - 0 (IK protein, human)
RN  - 0 (Ik protein, mouse)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 5/genetics
MH  - Cloning, Molecular
MH  - *Cytokines
MH  - Humans
MH  - Mice
MH  - Nuclear Proteins/chemistry/*genetics
MH  - RNA, Messenger/metabolism
MH  - *Repetitive Sequences, Amino Acid
MH  - Sequence Alignment
MH  - Sequence Deletion
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/04/27 00:00
MHDA- 1999/04/27 00:01
CRDT- 1999/04/27 00:00
PHST- 1999/04/27 00:00 [pubmed]
PHST- 1999/04/27 00:01 [medline]
PHST- 1999/04/27 00:00 [entrez]
AID - S0378111999000669 [pii]
AID - 10.1016/s0378-1119(99)00066-9 [doi]
PST - ppublish
SO  - Gene. 1999 Apr 16;230(2):145-54. doi: 10.1016/s0378-1119(99)00066-9.