PMID- 10215863
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 261
IP  - 2
DP  - 1999 Apr
TI  - Identification of a novel human adenylate kinase. cDNA cloning, expression
      analysis, chromosome localization and characterization of the recombinant
      protein.
PG  - 509-17
AB  - Adenylate kinases have an important role in the synthesis of adenine nucleotides 
      that are required for cellular metabolism. We report the cDNA cloning of a novel 
      22-kDa human enzyme that is sequence related to the human adenylate kinases and
      to UMP/CMP kinase of several species. The enzyme was expressed in Escherichia
      coli and shown to catalyse phosphorylation of AMP and dAMP with ATP as phosphate 
      donor. When GTP was used as phosphate donor, the enzyme phosphorylated AMP, CMP, 
      and to a small extent dCMP. Expression as a fusion protein with the green
      fluorescent protein showed that the enzyme is located in the cytosol. Northern
      blot analysis with mRNA from eight different human tissues demonstrated that the 
      enzyme was expressed exclusively in brain, with two mRNA isoforms of 2.4 and 4.0 
      kb. The gene that encoded the enzyme was localized to chromosome 1p31. Based on
      the substrate specificity and the sequence similarity with the previously
      identified human adenylate kinases, we have named this novel enzyme adenylate
      kinase 5.
FAU - Van Rompay, A R
AU  - Van Rompay AR
AD  - Division of Clinical Virology, Karolinska Institute, Huddinge University
      Hospital, Stockholm, Sweden.
FAU - Johansson, M
AU  - Johansson M
FAU - Karlsson, A
AU  - Karlsson A
LA  - eng
SI  - GENBANK/AF062595
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.4.3 (Adenylate Kinase)
RN  - EC 2.7.4.3 (adenylate kinase 5)
SB  - IM
MH  - Adenylate Kinase/biosynthesis/chemistry/*genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Humans
MH  - Isoenzymes/chemistry/*genetics
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Phylogeny
MH  - RNA, Messenger/metabolism
MH  - Recombinant Proteins/chemistry/genetics
MH  - Sequence Homology, Amino Acid
MH  - Substrate Specificity
MH  - Transfection
EDAT- 1999/04/24 00:00
MHDA- 1999/04/24 00:01
CRDT- 1999/04/24 00:00
PHST- 1999/04/24 00:00 [pubmed]
PHST- 1999/04/24 00:01 [medline]
PHST- 1999/04/24 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00294.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Apr;261(2):509-17. doi: 10.1046/j.1432-1327.1999.00294.x.