PMID- 10215861
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 261
IP  - 2
DP  - 1999 Apr
TI  - Alkyl-dihydroxyacetone phosphate synthase and dihydroxyacetone phosphate
      acyltransferase form a protein complex in peroxisomes.
PG  - 492-9
AB  - Dihydroxyacetone phosphate (GrnP) acyltransferase and alkyl-GrnP synthase are the
      key enzymes involved in the biosynthesis of ether phospholipids. Both enzymes are
      located on the inside of the peroxisomal membrane. Here we report evidence for a 
      direct interaction between these enzymes obtained by the use of chemical
      cross-linking. After cross-linking and immunoblot analysis alkyl-GrnP synthase
      could be detected in a 210-kDa complex which was located entirely on the lumenal 
      side of the peroxisomal membrane. Two-dimensional SDS/PAGE demonstrated that
      GrnP-acyltransferase is also cross-linked in a 210-kDa complex.
      Co-immunoprecipitation confirmed that the two enzymes interact, in a
      heterotrimeric complex. Furthermore, alkyl-GrnP synthase can form a homotrimeric 
      complex in the absence of GrnP-acyltransferase as was demonstrated by immunoblot 
      analysis after cross-linking experiments with either GrnP-acyltransferase
      deficient human fibroblast homogenates or recombinant (His)6-tagged alkyl-GrnP
      synthase. We conclude that alkyl-GrnP synthase interacts selectively with
      GrnP-acyltransferase in a heterotrimeric complex and in the absence of
      GrnP-acyltransferase can also form a homotrimeric complex.
FAU - Biermann, J
AU  - Biermann J
AD  - Centre for Biomembranes and Lipid Enzymology, Institut for Biomembranes, Utrecht 
      University, The Netherlands.
FAU - Just, W W
AU  - Just WW
FAU - Wanders, R J
AU  - Wanders RJ
FAU - Van Den Bosch, H
AU  - Van Den Bosch H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.42 (glycerone-phosphate O-acyltransferase)
RN  - EC 2.5.- (Alkyl and Aryl Transferases)
RN  - EC 2.5.1.26 (alkylglycerone-phosphate synthase)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Acyltransferases/deficiency/*metabolism
MH  - Alkyl and Aryl Transferases/*metabolism
MH  - Animals
MH  - Fibroblasts
MH  - Guinea Pigs
MH  - Humans
MH  - Immunoblotting
MH  - Liver/*enzymology
MH  - Microbodies/*enzymology
MH  - Precipitin Tests
MH  - Protein Conformation
MH  - Trypsin/metabolism
EDAT- 1999/04/24 00:00
MHDA- 1999/04/24 00:01
CRDT- 1999/04/24 00:00
PHST- 1999/04/24 00:00 [pubmed]
PHST- 1999/04/24 00:01 [medline]
PHST- 1999/04/24 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00295.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Apr;261(2):492-9. doi: 10.1046/j.1432-1327.1999.00295.x.