PMID- 10215321
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20161019
IS  - 1046-6673 (Print)
IS  - 1046-6673 (Linking)
VI  - 10
IP  - 2
DP  - 1999 Feb
TI  - Cloning and characterization of two new isoforms of the rat kidney urea
      transporter: UT-A3 and UT-A4.
PG  - 230-7
AB  - Urea transport in the kidney is important for the production of concentrated
      urine and is mediated by a family of transporter proteins, identified from
      erythropoietic tissue (UT-B) and from kidney (UT-A). Two isoforms of the renal
      urea transporter (UT-A) have been cloned so far: UT-A1 and UT-A2. We used rapid
      amplification of cDNA ends to clone two new isoforms of the rat UT-A transporter:
      UT-A3 and UT-A4. UT-A3 and UT-A4 are 87% homologous. The UT-A3 cDNA encodes a
      peptide of 460 amino acids, which corresponds to the amino-terminal half of the
      UT-A1 peptide and is 62% identical to UT-A2. The UT-A4 cDNA encodes a peptide of 
      466 amino acids, which is 84% identical to UT-A2. Transient transfection of
      HEK-293 cells with the UT-A3 or UT-A4 cDNA results in phloretin-inhibitable urea 
      uptake, which is increased by forskolin. Thus, both new isoforms encode
      functional urea transporters that may be vasopressin-regulated. UT-A3 and UT-A4
      mRNA are expressed in the renal outer and inner medulla but not in the cortex;
      unidentified UT-A isoforms similar to UT-A3 may also be expressed in the testis. 
      It is concluded that there are at least four different rat UT-A urea
      transporters.
FAU - Karakashian, A
AU  - Karakashian A
AD  - Department of Pathology, Emory University School of Medicine, Atlanta, Georgia
      30322, USA.
FAU - Timmer, R T
AU  - Timmer RT
FAU - Klein, J D
AU  - Klein JD
FAU - Gunn, R B
AU  - Gunn RB
FAU - Sands, J M
AU  - Sands JM
FAU - Bagnasco, S M
AU  - Bagnasco SM
LA  - eng
SI  - GENBANK/AF041788
SI  - GENBANK/AF042167
GR  - R01 DK041707/DK/NIDDK NIH HHS/United States
GR  - P01-DK50268/DK/NIDDK NIH HHS/United States
GR  - R01-DK41707/DK/NIDDK NIH HHS/United States
GR  - R37HL28674/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Am Soc Nephrol
JT  - Journal of the American Society of Nephrology : JASN
JID - 9013836
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (urea transporter)
SB  - IM
MH  - Amino Acid Sequence/genetics
MH  - Animals
MH  - Base Sequence/genetics
MH  - Carrier Proteins/*genetics
MH  - Cell Line
MH  - *Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Humans
MH  - Kidney/cytology/embryology/metabolism
MH  - Membrane Glycoproteins/*genetics
MH  - *Membrane Transport Proteins
MH  - Molecular Sequence Data
MH  - Protein Biosynthesis/genetics
MH  - RNA, Messenger/metabolism
MH  - Rats/*genetics
MH  - Tissue Distribution
EDAT- 1999/04/24 00:00
MHDA- 1999/04/24 00:01
CRDT- 1999/04/24 00:00
PHST- 1999/04/24 00:00 [pubmed]
PHST- 1999/04/24 00:01 [medline]
PHST- 1999/04/24 00:00 [entrez]
PST - ppublish
SO  - J Am Soc Nephrol. 1999 Feb;10(2):230-7.