PMID- 10214964
OWN - NLM
STAT- MEDLINE
DCOM- 19990505
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 447
IP  - 2-3
DP  - 1999 Mar 26
TI  - Identification of the sequence responsible for the nuclear localization of human 
      Cdc6.
PG  - 292-6
AB  - The Cdc6 is the essential protein for the initiation of DNA replication. Cdc6 is 
      localized in the G1 nucleus, and abnormal nuclear localization of this protein
      induces irregular initiation of DNA replication. We identified here that amino
      acids K57 and R58 in the human Cdc6 protein play an important role in the nuclear
      localization of the protein. The fundamental features of the mechanism regulating
      the localization of Cdc6 seem to be maintained in yeast, Xenopus, and human,
      since the amino acid sequence surrounding K57 and R58, (S/T)PXKR(L/I), is
      conserved in these species. Substitution of amino acid residue S54 with E and not
      Q blocked partially the nuclear localization of the protein, implying that the
      phosphorylation at S54 is involved in the regulating mechanism of the cell
      cycle-dependent localization of Cdc6.
FAU - Takei, Y
AU  - Takei Y
AD  - Division of Molecular Cell Pharmacology, National Children's Medical Research
      Center, Tokyo, Japan.
FAU - Yamamoto, K
AU  - Yamamoto K
FAU - Tsujimoto, G
AU  - Tsujimoto G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (CDC6 protein, S cerevisiae)
RN  - 0 (CDC6 protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fungal Proteins)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - Cell Cycle Proteins/chemistry/*genetics/metabolism
MH  - Conserved Sequence
MH  - DNA Primers/genetics
MH  - DNA Replication
MH  - DNA, Complementary/genetics
MH  - Fungal Proteins/genetics
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nuclear Localization Signals/*genetics
MH  - Nuclear Proteins/chemistry/*genetics/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - *Saccharomyces cerevisiae Proteins
MH  - Schizosaccharomyces/genetics
MH  - Sequence Homology, Amino Acid
MH  - Xenopus laevis
EDAT- 1999/04/24 00:00
MHDA- 1999/04/24 00:01
CRDT- 1999/04/24 00:00
PHST- 1999/04/24 00:00 [pubmed]
PHST- 1999/04/24 00:01 [medline]
PHST- 1999/04/24 00:00 [entrez]
AID - S0014-5793(99)00306-3 [pii]
AID - 10.1016/s0014-5793(99)00306-3 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Mar 26;447(2-3):292-6. doi: 10.1016/s0014-5793(99)00306-3.