PMID- 10214954
OWN - NLM
STAT- MEDLINE
DCOM- 19990505
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 447
IP  - 2-3
DP  - 1999 Mar 26
TI  - Biochemical analysis of interleukin-2 receptor beta chain phosphorylation by
      p56(lck).
PG  - 241-6
AB  - Tyrosine phosphorylation of multiple proteins, including the receptor itself, is 
      an initial event in IL-2 signaling and leads to recruitment of SH2 or PTB
      domain-containing proteins to the receptor. In this study, we have used
      subdomains of the IL-2 receptor beta chain (IL-2Rbeta) expressed in Escherichia
      coli as GST fusion proteins to identify the tyrosine residues that could be
      phosphorylated by p56(lck), one of the critical tyrosine kinases activated by
      IL-2. We report that recombinant p56(lck) phosphorylates in vitro tyrosine
      residues within the IL-2Rbeta chain but not those within the IL-2Rgamma chain.
      p56(lck) phosphorylates tyrosine residues 355, 358 and 361 but not 338 of the
      IL-2Rbeta chain acidic subdomain. Interestingly, phosphorylation of Tyr-358
      appears to require the presence of either Tyr-355 or Tyr-361. p56(lck) also
      phosphorylates very efficiently the two tyrosines present in the IL-2Rbeta chain 
      C-terminal region, Tyr-392 and Tyr-510. We also investigated the association of
      p56(lck) with the IL-2Rbeta chain which was found to depend on a short stretch of
      the IL-2Rbeta chain acidic subdomain, and to be independent of the presence of
      its tyrosine residues.
FAU - Delespine-Carmagnat, M
AU  - Delespine-Carmagnat M
AD  - INSERM Unit 461, Faculte de Pharmacie Paris-XI, Chatenay-Malabry, France.
FAU - Bouvier, G
AU  - Bouvier G
FAU - Allee, G
AU  - Allee G
FAU - Fagard, R
AU  - Fagard R
FAU - Bertoglio, J
AU  - Bertoglio J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA Primers)
RN  - 0 (Receptors, Interleukin-2)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.10.2 (Lymphocyte Specific Protein Tyrosine Kinase p56(lck))
SB  - IM
MH  - Base Sequence
MH  - Binding Sites
MH  - DNA Primers/genetics
MH  - Escherichia coli/genetics
MH  - Humans
MH  - In Vitro Techniques
MH  - Lymphocyte Specific Protein Tyrosine Kinase p56(lck)/genetics/*metabolism
MH  - Phosphorylation
MH  - Protein Conformation
MH  - Receptors, Interleukin-2/chemistry/genetics/*metabolism
MH  - Recombinant Fusion Proteins/chemistry/genetics/metabolism
MH  - Signal Transduction
MH  - Tyrosine/chemistry/metabolism
EDAT- 1999/04/24 00:00
MHDA- 1999/04/24 00:01
CRDT- 1999/04/24 00:00
PHST- 1999/04/24 00:00 [pubmed]
PHST- 1999/04/24 00:01 [medline]
PHST- 1999/04/24 00:00 [entrez]
AID - S0014-5793(99)00301-4 [pii]
AID - 10.1016/s0014-5793(99)00301-4 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Mar 26;447(2-3):241-6. doi: 10.1016/s0014-5793(99)00301-4.