PMID- 10213689
OWN - NLM
STAT- MEDLINE
DCOM- 19990511
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 284
IP  - 5414
DP  - 1999 Apr 23
TI  - Fas-induced caspase denitrosylation.
PG  - 651-4
AB  - Only a few intracellular S-nitrosylated proteins have been identified, and it is 
      unknown if protein S-nitrosylation/denitrosylation is a component of signal
      transduction cascades. Caspase-3 zymogens were found to be S-nitrosylated on
      their catalytic-site cysteine in unstimulated human cell lines and denitrosylated
      upon activation of the Fas apoptotic pathway. Decreased caspase-3 S-nitrosylation
      was associated with an increase in intracellular caspase activity. Fas therefore 
      activates caspase-3 not only by inducing the cleavage of the caspase zymogen to
      its active subunits, but also by stimulating the denitrosylation of its
      active-site thiol. Protein S-nitrosylation/denitrosylation can thus serve as a
      regulatory process in signal transduction pathways.
FAU - Mannick, J B
AU  - Mannick JB
AD  - Department of Adult Oncology, Dana Farber Cancer Institute, 44 Binney Street,
      Boston, MA 02115, USA. joan_mannick@dfci.harvard.edu
FAU - Hausladen, A
AU  - Hausladen A
FAU - Liu, L
AU  - Liu L
FAU - Hess, D T
AU  - Hess DT
FAU - Zeng, M
AU  - Zeng M
FAU - Miao, Q X
AU  - Miao QX
FAU - Kane, L S
AU  - Kane LS
FAU - Gow, A J
AU  - Gow AJ
FAU - Stamler, J S
AU  - Stamler JS
LA  - eng
GR  - GM57601-01/GM/NIGMS NIH HHS/United States
GR  - HL52529/HL/NHLBI NIH HHS/United States
GR  - HL59130/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Enzyme Precursors)
RN  - 0 (Nitrites)
RN  - 0 (Nitroso Compounds)
RN  - 0 (S-Nitrosothiols)
RN  - 0 (fas Receptor)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 60-24-2 (Mercaptoethanol)
RN  - 67616-44-8 (S-nitrosomercaptoethanol)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Binding Sites
MH  - Caspase 3
MH  - Caspases/*metabolism
MH  - Cell Line
MH  - Cysteine/*metabolism
MH  - Enzyme Activation
MH  - Enzyme Inhibitors/pharmacology
MH  - Enzyme Precursors/metabolism
MH  - Humans
MH  - *Mercaptoethanol
MH  - Nitric Oxide/*metabolism
MH  - Nitric Oxide Synthase/antagonists & inhibitors
MH  - Nitrites/metabolism
MH  - Nitroso Compounds/metabolism
MH  - *S-Nitrosothiols
MH  - Signal Transduction
MH  - fas Receptor/*physiology
MH  - omega-N-Methylarginine/pharmacology
EDAT- 1999/04/24 00:00
MHDA- 1999/04/24 00:01
CRDT- 1999/04/24 00:00
PHST- 1999/04/24 00:00 [pubmed]
PHST- 1999/04/24 00:01 [medline]
PHST- 1999/04/24 00:00 [entrez]
AID - 10.1126/science.284.5414.651 [doi]
PST - ppublish
SO  - Science. 1999 Apr 23;284(5414):651-4. doi: 10.1126/science.284.5414.651.