PMID- 10213386
OWN - NLM
STAT- MEDLINE
DCOM- 19990709
LR  - 20191210
IS  - 1058-8388 (Print)
IS  - 1058-8388 (Linking)
VI  - 214
IP  - 4
DP  - 1999 Apr
TI  - Murine Gcm1 gene is expressed in a subset of placental trophoblast cells.
PG  - 303-11
AB  - The gcm gene of Drosophila melanogaster encodes a transcription factor that is an
      important component in cell fate specification within the nervous system. In the 
      absence of a functional gcm gene, progenitor cells differentiate into neurons,
      whereas when the gene is ectopically expressed the cells produce excess glial
      cells at the expense of neuronal differentiation. Recent searches of databases
      have uncovered high sequence similarity between the Drosophila gem gene and an
      anonymous human placental cDNA clone (Altschuller et al., 1996; this
      communication). Here we report the molecular organization of the murine Gcm1, its
      spatio-temporal pattern of expression in developing placenta, and its map
      position at E1-E3 on murine chromosome 9. The murine gene is composed of at least
      6 exons. The promoter region contains an "initiation sequence" and is GC rich,
      characteristics of the promoters of several transcription factors. The mRNA has a
      modest 5'UTR (ca. 200 bases) but an extensive 3' UTR (ca. 2 kb). Northern blot
      and mRNA in situ hybridization studies showed that Gcm1 expression was readily
      detectable only in the placenta. It began at embryonic day 7.5 within trophoblast
      cells of the chorion and continued to about embryonic day 17.5 within a subset of
      labyrinthine trophoblast cells. Comparison with other transcription factors
      revealed that Gcm1 expression defines a unique subset of trophoblast cells.
FAU - Basyuk, E
AU  - Basyuk E
AD  - Brookdale Center for Developmental and Molecular Biology, Mount Sinai School of
      Medicine, New York, New York 10029, USA.
FAU - Cross, J C
AU  - Cross JC
FAU - Corbin, J
AU  - Corbin J
FAU - Nakayama, H
AU  - Nakayama H
FAU - Hunter, P
AU  - Hunter P
FAU - Nait-Oumesmar, B
AU  - Nait-Oumesmar B
FAU - Lazzarini, R A
AU  - Lazzarini RA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Dev Dyn
JT  - Developmental dynamics : an official publication of the American Association of
      Anatomists
JID - 9201927
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Esrrb protein, mouse)
RN  - 0 (GCM1 protein, human)
RN  - 0 (Gcm1 protein, mouse)
RN  - 0 (Homeobox A10 Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Transcription Factors)
RN  - 140441-81-2 (HOXA10 protein, human)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Chromosome Mapping
MH  - DNA-Binding Proteins/metabolism
MH  - Exons
MH  - Expressed Sequence Tags
MH  - Gene Expression Regulation, Developmental
MH  - Gene Library
MH  - Homeobox A10 Proteins
MH  - Homeodomain Proteins
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Introns
MH  - Mice
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Neuropeptides/*metabolism
MH  - Nuclear Proteins
MH  - Placenta/anatomy & histology/*metabolism
MH  - Receptors, Cytoplasmic and Nuclear/metabolism
MH  - Receptors, Estrogen/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - Transcription Factors/metabolism
MH  - Trophoblasts/*metabolism
EDAT- 1999/04/23 02:03
MHDA- 2000/06/22 10:00
CRDT- 1999/04/23 02:03
PHST- 1999/04/23 02:03 [pubmed]
PHST- 2000/06/22 10:00 [medline]
PHST- 1999/04/23 02:03 [entrez]
AID - 10.1002/(SICI)1097-0177(199904)214:4<303::AID-AJA3>3.0.CO;2-B [pii]
AID - 10.1002/(SICI)1097-0177(199904)214:4<303::AID-AJA3>3.0.CO;2-B [doi]
PST - ppublish
SO  - Dev Dyn. 1999 Apr;214(4):303-11. doi:
      10.1002/(SICI)1097-0177(199904)214:4<303::AID-AJA3>3.0.CO;2-B.