PMID- 10213384
OWN - NLM
STAT- MEDLINE
DCOM- 19990709
LR  - 20191210
IS  - 1058-8388 (Print)
IS  - 1058-8388 (Linking)
VI  - 214
IP  - 4
DP  - 1999 Apr
TI  - Maturational disturbance of chondrocytes in Cbfa1-deficient mice.
PG  - 279-90
AB  - Cbfa1, a transcription factor that belongs to the runt-domain gene family, plays 
      an essential role in osteogenesis. Cbfa1-deficient mice completely lacked both
      intramembranous and endochondral ossification, owing to the maturational arrest
      of osteoblasts, indicating that Cbfa1 has a fundamental role in osteoblast
      differentiation. However, Cbfa1 was also expressed in chondrocytes, and its
      expression was increased according to the maturation of chondrocytes. Terminal
      hypertrophic chondrocytes expressed Cbfa1 extensively. The significant expression
      of Cbfa1 in hypertrophic chondrocytes was first detected at embryonic day 13.5
      (E13.5), and its expression in hypertrophic chondrocytes was most prominent at
      E14.5-16.5. In Cbfa1-deficient mice, whose entire skeleton was composed of
      cartilage, the chondrocyte differentiation was disturbed. Calcification of
      cartilage occurred in the restricted parts of skeletons, including tibia, fibula,
      radius, and ulna. Type X collagen, BMP6, and Indian hedgehog were expressed in
      their hypertrophic chondrocytes. However, osteopontin, bone sialoprotein, and
      collagenase 3 were not expressed at all, indicating that they are directly
      regulated by Cbfa1 in the terminal hypertrophic chondrocytes. Chondrocyte
      differentiation was severely disturbed in the rest of the skeleton. The
      expression of PTH/PTHrP receptor, Indian hedgehog, type X collagen, and BMP6 was 
      not detected in humerus and femur, indicating that chondrocyte differentiation
      was blocked before prehypertrophic chondrocytes. These findings demonstrate that 
      Cbfa1 is an important factor for chondrocyte differentiation.
FAU - Inada, M
AU  - Inada M
AD  - Department of Medicine III, Osaka University Medical School, Japan.
FAU - Yasui, T
AU  - Yasui T
FAU - Nomura, S
AU  - Nomura S
FAU - Miyake, S
AU  - Miyake S
FAU - Deguchi, K
AU  - Deguchi K
FAU - Himeno, M
AU  - Himeno M
FAU - Sato, M
AU  - Sato M
FAU - Yamagiwa, H
AU  - Yamagiwa H
FAU - Kimura, T
AU  - Kimura T
FAU - Yasui, N
AU  - Yasui N
FAU - Ochi, T
AU  - Ochi T
FAU - Endo, N
AU  - Endo N
FAU - Kitamura, Y
AU  - Kitamura Y
FAU - Kishimoto, T
AU  - Kishimoto T
FAU - Komori, T
AU  - Komori T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Dyn
JT  - Developmental dynamics : an official publication of the American Association of
      Anatomists
JID - 9201927
RN  - 0 (Bmp6 protein, mouse)
RN  - 0 (Bone Morphogenetic Protein 6)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Cnmd protein, mouse)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Growth Substances)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Parathyroid Hormone)
RN  - 0 (Parathyroid Hormone-Related Protein)
RN  - 0 (Proteins)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (Spp1 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0C2P5QKL36 (Sulfobromophthalein)
RN  - 106441-73-0 (Osteopontin)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.- (Mmp13 protein, mouse)
SB  - IM
SB  - S
MH  - Animals
MH  - Blotting, Northern
MH  - Bone Morphogenetic Protein 6
MH  - Bone Morphogenetic Proteins/metabolism
MH  - Cartilage/anatomy & histology/blood supply
MH  - Cell Division
MH  - Chondrocytes/*physiology
MH  - Collagen/metabolism
MH  - Collagenases/metabolism
MH  - Core Binding Factor Alpha 1 Subunit
MH  - Embryo, Mammalian/anatomy & histology
MH  - Femur/embryology
MH  - Gene Expression Regulation, Developmental
MH  - Growth Substances/metabolism
MH  - Hedgehog Proteins
MH  - Humerus/embryology
MH  - In Situ Hybridization
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Matrix Metalloproteinase 13
MH  - *Membrane Proteins
MH  - Mice
MH  - *Neoplasm Proteins
MH  - Osteoblasts/physiology
MH  - Osteopontin
MH  - Parathyroid Hormone/metabolism
MH  - Parathyroid Hormone-Related Protein
MH  - Proteins/metabolism
MH  - Radius/embryology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sialoglycoproteins/metabolism
MH  - Sulfobromophthalein/metabolism
MH  - Tibia/embryology
MH  - *Trans-Activators
MH  - Transcription Factors/*deficiency
EDAT- 1999/04/23 02:03
MHDA- 2000/06/22 10:00
CRDT- 1999/04/23 02:03
PHST- 1999/04/23 02:03 [pubmed]
PHST- 2000/06/22 10:00 [medline]
PHST- 1999/04/23 02:03 [entrez]
AID - 10.1002/(SICI)1097-0177(199904)214:4<279::AID-AJA1>3.0.CO;2-W [pii]
AID - 10.1002/(SICI)1097-0177(199904)214:4<279::AID-AJA1>3.0.CO;2-W [doi]
PST - ppublish
SO  - Dev Dyn. 1999 Apr;214(4):279-90. doi:
      10.1002/(SICI)1097-0177(199904)214:4<279::AID-AJA1>3.0.CO;2-W.