PMID- 10213081
OWN - NLM
STAT- MEDLINE
DCOM- 19990616
LR  - 20191210
IS  - 0214-6282 (Print)
IS  - 0214-6282 (Linking)
VI  - 43
IP  - 1
DP  - 1999 Jan
TI  - Spatiotemporal pattern of the mouse chondromodulin-I gene expression and its
      regulatory role in vascular invasion into cartilage during endochondral bone
      formation.
PG  - 39-49
AB  - During endochondral bone formation, vascular invasion into cartilage initiates
      the replacement of cartilage by bone. Chondromodulin-I, a 25 kDa glycoprotein
      purified from bovine epiphyseal cartilage, was recently identified as a novel
      endothelial cell growth inhibitor. Here we cloned the mouse chondromodulin-I cDNA
      from a mouse whole embryo cDNA library. Northern blot analysis revealed that the 
      chondromodulin-I transcripts were expressed in association with the formation of 
      cartilage expressing type II collagen from days 11 to 17 of gestation in mouse
      embryos, at which time cartilaginous bone rudiments were gradually replaced by
      bone. Chondromodulin-I mRNA was also detected in the thymus and eyes at a lower
      level. In situ hybridization revealed significant expression in all cartilaginous
      tissues in the embryos at days 13.5 and 16 of gestation. However, the expression 
      was completely abolished in the hypertrophic cartilage zone prior to
      calcification. Upon chondrogenic differentiation of mouse ATDC5 cells in vitro,
      the expression of chondromodulin-I transcripts was induced concomitantly with the
      formation of type II collagen-expressing chondrocytes. The expression of the
      transcripts then declined as type X collagen-expressing hypertrophic chondrocytes
      appeared in the culture. Purified chondromodulin-I protein inhibited the vascular
      invasion into cartilage ectopically induced by demineralized bone matrix in nude 
      mice, leading to the suppression of bone formation in vivo. These results suggest
      that chondromodulin-I is involved in the anti-angiogenic property of cartilage,
      and that the withdrawal of its expression allows the vascular invasion which
      triggers the replacement of cartilage by bone during endochondral bone
      development.
FAU - Shukunami, C
AU  - Shukunami C
AD  - Department of Molecular Interaction and Tissue Engineering, Institute for
      Frontier Medical Sciences, Kyoto University, Japan.
FAU - Iyama, K
AU  - Iyama K
FAU - Inoue, H
AU  - Inoue H
FAU - Hiraki, Y
AU  - Hiraki Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - Int J Dev Biol
JT  - The International journal of developmental biology
JID - 8917470
RN  - 0 (Bmp2 protein, mouse)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Cnmd protein, mouse)
RN  - 0 (Growth Substances)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
SB  - S
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Bone Morphogenetic Protein 2
MH  - Bone Morphogenetic Proteins/metabolism
MH  - Bone and Bones/*embryology
MH  - Cartilage/*blood supply
MH  - Cattle
MH  - Cell Line
MH  - Choristoma/metabolism
MH  - Cloning, Molecular
MH  - Embryo, Mammalian/anatomy & histology/metabolism
MH  - Growth Substances/*genetics
MH  - In Situ Hybridization
MH  - *Intercellular Signaling Peptides and Proteins
MH  - *Membrane Proteins
MH  - Mice
MH  - Mice, Nude
MH  - Molecular Sequence Data
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Time Factors
MH  - Tissue Distribution
MH  - *Transforming Growth Factor beta
EDAT- 1999/04/23 00:00
MHDA- 1999/04/23 00:01
CRDT- 1999/04/23 00:00
PHST- 1999/04/23 00:00 [pubmed]
PHST- 1999/04/23 00:01 [medline]
PHST- 1999/04/23 00:00 [entrez]
PST - ppublish
SO  - Int J Dev Biol. 1999 Jan;43(1):39-49.