PMID- 10212280
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 18
DP  - 1999 Apr 30
TI  - Effects of overexpression of PTP36, a putative protein tyrosine phosphatase, on
      cell adhesion, cell growth, and cytoskeletons in HeLa cells.
PG  - 12905-9
AB  - Non-receptor-type putative protein tyrosine phosphatase-36 (PTP36), also known as
      PTPD2/Pez, possesses a domain homologous to the N-terminal half of band 4.1
      protein. To gain insight into the biological function of PTP36, we established a 
      HeLa cell line, HtTA/P36-9, in which the overexpression of PTP36 was inducible.
      PTP36 expressed in HeLa cells was enriched in the cytoskeleton near the plasma
      membrane. There was little endogenous PTP36 detectable in uninduced HtTA/P36-9
      cells or in the parental HeLa cells. Upon induction of PTP36 overexpression,
      HtTA/P36-9 cells spread less well, grew more slowly, and adhered to the
      extracellular matrix proteins less well than uninduced cells. Moreover, decreases
      in the actin stress fibers and the number of focal adhesions were observed. The
      tyrosine phosphorylation of the focal adhesion kinase induced by lysophosphatidic
      acid was suppressed in the HtTA/P36-9 cells overexpressing PTP36. These results
      indicate that PTP36 affects cytoskeletons, cell adhesion, and cell growth, thus
      suggesting that PTP36 is involved in their regulatory processes.
FAU - Ogata, M
AU  - Ogata M
AD  - Department of Oncology, Biomedical Research Center, Osaka University Medical
      School, Suita, Osaka 565-0871, Japan. mogata@ongene.med.osaka-u.ac.jp
FAU - Takada, T
AU  - Takada T
FAU - Mori, Y
AU  - Mori Y
FAU - Oh-hora, M
AU  - Oh-hora M
FAU - Uchida, Y
AU  - Uchida Y
FAU - Kosugi, A
AU  - Kosugi A
FAU - Miyake, K
AU  - Miyake K
FAU - Hamaoka, T
AU  - Hamaoka T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Extracellular Matrix Proteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Focal Adhesion Kinase 1)
RN  - EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (PTK2 protein, human)
RN  - EC 3.1.3.48 (PTPN14 protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases, Non-Receptor)
SB  - IM
MH  - *Cell Adhesion
MH  - Cell Adhesion Molecules/metabolism
MH  - *Cell Division
MH  - Cytoskeleton/*metabolism
MH  - Extracellular Matrix Proteins/metabolism
MH  - Focal Adhesion Kinase 1
MH  - Focal Adhesion Protein-Tyrosine Kinases
MH  - HeLa Cells
MH  - Humans
MH  - Phosphorylation
MH  - Protein Tyrosine Phosphatases/*metabolism
MH  - Protein Tyrosine Phosphatases, Non-Receptor
MH  - Protein-Tyrosine Kinases/metabolism
EDAT- 1999/04/23 00:00
MHDA- 1999/04/23 00:01
CRDT- 1999/04/23 00:00
PHST- 1999/04/23 00:00 [pubmed]
PHST- 1999/04/23 00:01 [medline]
PHST- 1999/04/23 00:00 [entrez]
AID - 10.1074/jbc.274.18.12905 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 30;274(18):12905-9. doi: 10.1074/jbc.274.18.12905.