PMID- 10212258
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 18
DP  - 1999 Apr 30
TI  - Radiation-induced assembly of Rad51 and Rad52 recombination complex requires ATM 
      and c-Abl.
PG  - 12748-52
AB  - Cells from individuals with the recessive cancer-prone disorder ataxia
      telangiectasia (A-T) are hypersensitive to ionizing radiation (I-R). ATM (mutated
      in A-T) is a protein kinase whose activity is stimulated by I-R. c-Abl, a
      nonreceptor tyrosine kinase, interacts with ATM and is activated by ATM following
      I-R. Rad51 is a homologue of bacterial RecA protein required for DNA
      recombination and repair. Here we demonstrate that there is an I-R-induced Rad51 
      tyrosine phosphorylation, and this induction is dependent on both ATM and c-Abl. 
      ATM, c-Abl, and Rad51 can be co-immunoprecipitated from cell extracts. Consistent
      with the physical interaction, c-Abl phosphorylates Rad51 in vitro and in vivo.
      In assays using purified components, phosphorylation of Rad51 by c-Abl enhances
      complex formation between Rad51 and Rad52, which cooperates with Rad51 in
      recombination and repair. After I-R, an increase in association between Rad51 and
      Rad52 occurs in wild-type cells but not in cells with mutations that compromise
      ATM or c-Abl. Our data suggest signaling mediated through ATM, and c-Abl is
      required for the correct post-translational modification of Rad51, which is
      critical for the assembly of Rad51 repair protein complex following I-R.
FAU - Chen, G
AU  - Chen G
AD  - Department of Molecular Medicine/Institute of Biotechnology, The University of
      Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA.
FAU - Yuan, S S
AU  - Yuan SS
FAU - Liu, W
AU  - Liu W
FAU - Xu, Y
AU  - Xu Y
FAU - Trujillo, K
AU  - Trujillo K
FAU - Song, B
AU  - Song B
FAU - Cong, F
AU  - Cong F
FAU - Goff, S P
AU  - Goff SP
FAU - Wu, Y
AU  - Wu Y
FAU - Arlinghaus, R
AU  - Arlinghaus R
FAU - Baltimore, D
AU  - Baltimore D
FAU - Gasser, P J
AU  - Gasser PJ
FAU - Park, M S
AU  - Park MS
FAU - Sung, P
AU  - Sung P
FAU - Lee, E Y
AU  - Lee EY
LA  - eng
GR  - 1RO1NS378381-01/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proteins)
RN  - 0 (Rad52 DNA Repair and Recombination Protein)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.11 (Checkpoint Kinase 2)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-abl)
RN  - EC 2.7.11.1 (Ataxia Telangiectasia Mutated Proteins)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.12.1 (RAD53 protein, S cerevisiae)
RN  - EC 2.7.7.- (Rad51 Recombinase)
SB  - IM
MH  - Ataxia Telangiectasia Mutated Proteins
MH  - *Cell Cycle Proteins
MH  - Checkpoint Kinase 2
MH  - DNA-Binding Proteins/*metabolism
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein Kinases/*metabolism
MH  - Protein Processing, Post-Translational
MH  - *Protein-Serine-Threonine Kinases
MH  - Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-abl/*metabolism
MH  - Rad51 Recombinase
MH  - Rad52 DNA Repair and Recombination Protein
MH  - *Recombination, Genetic
MH  - *Saccharomyces cerevisiae Proteins
MH  - Tumor Suppressor Proteins
MH  - Tyrosine/metabolism
EDAT- 1999/04/23 00:00
MHDA- 1999/04/23 00:01
CRDT- 1999/04/23 00:00
PHST- 1999/04/23 00:00 [pubmed]
PHST- 1999/04/23 00:01 [medline]
PHST- 1999/04/23 00:00 [entrez]
AID - 10.1074/jbc.274.18.12748 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 30;274(18):12748-52. doi: 10.1074/jbc.274.18.12748.