PMID- 10212242
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 18
DP  - 1999 Apr 30
TI  - Stress-activated protein kinase-3 interacts with the PDZ domain of
      alpha1-syntrophin. A mechanism for specific substrate recognition.
PG  - 12626-31
AB  - Mechanisms for selective targeting to unique subcellular sites play an important 
      role in determining the substrate specificities of protein kinases. Here we show 
      that stress-activated protein kinase-3 (SAPK3, also called ERK6 and p38gamma), a 
      member of the mitogen-activated protein kinase family that is abundantly
      expressed in skeletal muscle, binds through its carboxyl-terminal sequence -KETXL
      to the PDZ domain of alpha1-syntrophin. SAPK3 phosphorylates alpha1-syntrophin at
      serine residues 193 and 201 in vitro and phosphorylation is dependent on binding 
      to the PDZ domain of alpha1-syntrophin. In skeletal muscle SAPK3 and
      alpha1-syntrophin co-localize at the neuromuscular junction, and both proteins
      can be co-immunoprecipitated from transfected COS cell lysates. Phosphorylation
      of a PDZ domain-containing protein by an associated protein kinase is a novel
      mechanism for determining both the localization and the substrate specificity of 
      a protein kinase.
FAU - Hasegawa, M
AU  - Hasegawa M
AD  - Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge
      CB2 2QH, United Kingdom.
FAU - Cuenda, A
AU  - Cuenda A
FAU - Spillantini, M G
AU  - Spillantini MG
FAU - Thomas, G M
AU  - Thomas GM
FAU - Buee-Scherrer, V
AU  - Buee-Scherrer V
FAU - Cohen, P
AU  - Cohen P
FAU - Goedert, M
AU  - Goedert M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Bungarotoxins)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (syntrophin alpha1)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.- (Mitogen-Activated Protein Kinase 12)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bungarotoxins/metabolism
MH  - COS Cells
MH  - Calcium-Binding Proteins
MH  - Fluorescent Antibody Technique
MH  - Membrane Proteins/chemistry/*metabolism
MH  - Mitogen-Activated Protein Kinase 12
MH  - *Mitogen-Activated Protein Kinases
MH  - Molecular Sequence Data
MH  - Muscle Proteins/chemistry/*metabolism
MH  - Muscle, Skeletal/enzymology/metabolism
MH  - Neuromuscular Junction/enzymology/metabolism
MH  - Peptide Fragments/chemistry/metabolism
MH  - Phosphorylation
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Protein Kinases/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Substrate Specificity
EDAT- 1999/04/23 00:00
MHDA- 1999/04/23 00:01
CRDT- 1999/04/23 00:00
PHST- 1999/04/23 00:00 [pubmed]
PHST- 1999/04/23 00:01 [medline]
PHST- 1999/04/23 00:00 [entrez]
AID - 10.1074/jbc.274.18.12626 [doi]
AID - S0021-9258(19)73397-3 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 30;274(18):12626-31. doi: 10.1074/jbc.274.18.12626.