PMID- 10212226
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20191210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 18
DP  - 1999 Apr 30
TI  - Cloning, expression, and characterization of a novel
      UDP-galactose:beta-N-acetylglucosamine beta1,3-galactosyltransferase
      (beta3Gal-T5) responsible for synthesis of type 1 chain in colorectal and
      pancreatic epithelia and tumor cells derived therefrom.
PG  - 12499-507
AB  - The sialyl Lewis a antigen is a well known tumor marker, CA19-9, which is
      frequently elevated in the serum in gastrointestinal and pancreatic cancers.
      UDP-galactose:N-acetylglucosamine beta1, 3-galactosyltransferase(s) (beta3Gal-Ts)
      are required for the synthesis of the sialyl Lewis a epitope. In the present
      study, a novel beta3Gal-T, named beta3Gal-T5, was isolated from a Colo205 cDNA
      library using a degenerate primer strategy based on the amino acid sequences of
      the four human beta3Gal-T genes cloned to date. Transfection experiments
      demonstrated that HCT-15 cells transfected with the beta3Gal-T5 gene expressed
      all the type 1 Lewis antigens. In gastrointestinal and pancreatic cancer cell
      lines, the amounts of beta3Gal-T5 transcripts were quite well correlated with the
      amounts of the sialyl Lewis a antigens. The beta1,3Gal-T activity toward
      agalacto-lacto-N-neotetraose was also well correlated with the amounts of
      beta3Gal-T5 transcripts in a series of cultured cancer cells, and in Namalwa and 
      HCT-15 cells transfected with the beta3Gal-T5 gene. Thus, the beta3Gal-T5 gene is
      the most probable candidate responsible for the synthesis of the type 1 Lewis
      antigens in gastrointestinal and pancreatic epithelia and tumor cells derived
      therefrom. In addition, beta3Gal-T5 is a key enzyme that determines the amounts
      of the type 1 Lewis antigens including the sialyl Lewis a antigen.
FAU - Isshiki, S
AU  - Isshiki S
AD  - Division of Cell Biology, Institute of Life Science, Soka University, 1-236
      Tangi-cho, Hachioji, Tokyo 192-8577, Japan.
FAU - Togayachi, A
AU  - Togayachi A
FAU - Kudo, T
AU  - Kudo T
FAU - Nishihara, S
AU  - Nishihara S
FAU - Watanabe, M
AU  - Watanabe M
FAU - Kubota, T
AU  - Kubota T
FAU - Kitajima, M
AU  - Kitajima M
FAU - Shiraishi, N
AU  - Shiraishi N
FAU - Sasaki, K
AU  - Sasaki K
FAU - Andoh, T
AU  - Andoh T
FAU - Narimatsu, H
AU  - Narimatsu H
LA  - eng
SI  - GENBANK/AB020337
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CA-19-9 Antigen)
RN  - 0 (DNA, Complementary)
RN  - 0 (Gangliosides)
RN  - 0 (RNA, Messenger)
RN  - 91847-18-6 (sialyl Le(a) ganglioside)
RN  - EC 2.7.8.- (Transferases (Other Substituted Phosphate Groups))
RN  - EC 2.7.8.18 (UDPgalactose - UDP-N-acetylglucosamine galactosephosphotransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - CA-19-9 Antigen
MH  - Cloning, Molecular
MH  - Colorectal Neoplasms/*enzymology/immunology/pathology
MH  - DNA, Complementary
MH  - Gangliosides/immunology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Pancreatic Neoplasms/*enzymology/immunology/pathology
MH  - RNA, Messenger/genetics
MH  - Sequence Homology, Amino Acid
MH  - Transferases (Other Substituted Phosphate Groups)/*genetics/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1999/04/23 00:00
MHDA- 1999/04/23 00:01
CRDT- 1999/04/23 00:00
PHST- 1999/04/23 00:00 [pubmed]
PHST- 1999/04/23 00:01 [medline]
PHST- 1999/04/23 00:00 [entrez]
AID - 10.1074/jbc.274.18.12499 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 30;274(18):12499-507. doi: 10.1074/jbc.274.18.12499.