PMID- 10212223
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20220309
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 18
DP  - 1999 Apr 30
TI  - A receptor-like protein-tyrosine phosphatase PTPzeta/RPTPbeta binds a
      heparin-binding growth factor midkine. Involvement of arginine 78 of midkine in
      the high affinity binding to PTPzeta.
PG  - 12474-9
AB  - Midkine is a 13-kDa heparin-binding growth factor with 45% sequence identity to
      pleiotrophin. Pleiotrophin has been demonstrated to bind to protein-tyrosine
      phosphatase zeta (PTPzeta) with high affinity. In this study, we examined the
      binding of midkine to PTPzeta by solid-phase binding assay. Midkine and
      pleiotrophin binding to PTPzeta were equally inhibited by soluble pleiotrophin
      and also by some specific glycosaminoglycans. For both bindings, Scatchard
      analysis revealed low (3.0 nM) and high (0.58 nM) affinity binding sites. These
      results suggested that PTPzeta is a common receptor for midkine and pleiotrophin.
      Midkine is structurally divided into the N- and C-terminal halves, and the latter
      exhibited full activity for PTPzeta binding and neuronal migration induction. The
      C-terminal half contains two heparin-binding sites consisting of clusters of
      basic amino acids, Clusters I and II. A mutation at Arg78 in Cluster I resulted
      in loss of the high affinity binding and reduced neuronal migration-inducing
      activity, while mutations at Lys83 and Lys84 in Cluster II showed almost no
      effect on either activity. Chondroitinase ABC-treated PTPzeta exhibited similar
      low affinity binding both to the native midkine and midkine mutants at Arg78.
      These results suggested that Arg78 in midkine plays an essential role in high
      affinity binding to PTPzeta by interacting with the chondroitin sulfate portion
      of this receptor.
FAU - Maeda, N
AU  - Maeda N
AD  - Division of Molecular Neurobiology, National Institute for Basic Biology,
      Graduate University for Advanced Studies, Okazaki 444-8585, Japan.
FAU - Ichihara-Tanaka, K
AU  - Ichihara-Tanaka K
FAU - Kimura, T
AU  - Kimura T
FAU - Kadomatsu, K
AU  - Kadomatsu K
FAU - Muramatsu, T
AU  - Muramatsu T
FAU - Noda, M
AU  - Noda M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytokines)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 137497-38-2 (Midkine)
RN  - 9005-49-6 (Heparin)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (Ptprz1 protein, mouse)
RN  - EC 3.1.3.48 (Receptor-Like Protein Tyrosine Phosphatases, Class 5)
SB  - IM
MH  - Animals
MH  - Arginine/*metabolism
MH  - Carrier Proteins/chemistry/*metabolism
MH  - Cell Movement/drug effects
MH  - *Cytokines
MH  - Glycosaminoglycans/pharmacology
MH  - Heparin/*metabolism
MH  - Mice
MH  - Midkine
MH  - Nerve Tissue Proteins/antagonists & inhibitors/*metabolism
MH  - Protein Binding
MH  - Protein Tyrosine Phosphatases/antagonists & inhibitors/*metabolism
MH  - Receptor-Like Protein Tyrosine Phosphatases, Class 5
MH  - Receptors, Cell Surface/*metabolism
EDAT- 1999/04/23 00:00
MHDA- 1999/04/23 00:01
CRDT- 1999/04/23 00:00
PHST- 1999/04/23 00:00 [pubmed]
PHST- 1999/04/23 00:01 [medline]
PHST- 1999/04/23 00:00 [entrez]
AID - 10.1074/jbc.274.18.12474 [doi]
AID - S0021-9258(19)73378-X [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 30;274(18):12474-9. doi: 10.1074/jbc.274.18.12474.