PMID- 10212192
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20191210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 18
DP  - 1999 Apr 30
TI  - Expression and functional role of the gamma subunit of the Na, K-ATPase in
      mammalian cells.
PG  - 12252-6
AB  - The functional role of the gamma subunit of the Na,K-ATPase was studied using rat
      gamma cDNA-transfected HEK-293 cells and an antiserum (gammaC33) specific for
      gamma. Although the sequence for gamma was verified and shown to be larger (7237 
      Da) than first reported, it still comprises a single initiator methionine despite
      the expression of a gammaC33-reactive doublet on immunoblots. Kinetic analysis of
      the enzyme of transfected compared with control cells and of gammaC33-treated
      kidney pumps shows that gamma regulates the apparent affinity for ATP. Thus,
      gamma-transfected cells have a decreased K'ATP as shown in measurements of (i)
      K'ATP of Na,K-ATPase activity and (ii) K+ inhibition of Na-ATPase at 1 microM
      ATP. Consistent with the behavior of gamma-transfected cells, gammaC33
      pretreatment increases K'ATP of the kidney enzyme and K+ inhibition (1 microM
      ATP) of both kidney and gamma-transfected cells. These results are consistent
      with previous findings that an antiserum raised against the pig gamma subunit
      stabilizes the E2(K) form of the enzyme (Therien, A. G., Goldshleger, R.,
      Karlish, S. J., and Blostein, R. (1997) J. Biol. Chem. 272, 32628-32634).
      Overall, our data demonstrate that gamma is a tissue (kidney)-specific regulator 
      of the Na,K-ATPase that can increase the apparent affinity of the enzyme for ATP 
      in a manner that is reversible by anti-gamma antiserum.
FAU - Therien, A G
AU  - Therien AG
AD  - Department of Biochemistry, McGill University, Montreal, Quebec H3G 1A4, Canada.
FAU - Karlish, S J
AU  - Karlish SJ
FAU - Blostein, R
AU  - Blostein R
LA  - eng
SI  - GENBANK/AF129400
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA, Complementary
MH  - Humans
MH  - Kidney/enzymology
MH  - Molecular Sequence Data
MH  - Rats
MH  - Sodium-Potassium-Exchanging ATPase/chemistry/genetics/*metabolism
EDAT- 1999/04/23 00:00
MHDA- 1999/04/23 00:01
CRDT- 1999/04/23 00:00
PHST- 1999/04/23 00:00 [pubmed]
PHST- 1999/04/23 00:01 [medline]
PHST- 1999/04/23 00:00 [entrez]
AID - 10.1074/jbc.274.18.12252 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 30;274(18):12252-6. doi: 10.1074/jbc.274.18.12252.