PMID- 10211776
OWN - NLM
STAT- MEDLINE
DCOM- 19990607
LR  - 20190915
IS  - 0168-0102 (Print)
IS  - 0168-0102 (Linking)
VI  - 33
IP  - 2
DP  - 1999 Feb
TI  - Distinct spatiotemporal expression of mRNAs for the PSD-95/SAP90 protein family
      in the mouse brain.
PG  - 111-8
AB  - PSD-95 (SAP90), SAP102 and Chapsyn-110 (PSD-93) are members of the
      membrane-associated guanylate kinase family, and interact with
      N-methyl-D-aspartate (NMDA) receptor NR2A (GluRepsilon1) and NR2B (GluRepsilon2) 
      subunits and with Shaker-type K+ channel subunits to cluster into a channel
      complex. In the present study, we examined their expression in developing and
      adult mouse brains by in situ hybridization with antisense oligonucleotide
      probes. PSD-95 and SAP102 mRNAs were prominently expressed at embryonic day 13
      (E13) in the mantle zone of various brain regions, where NMDA receptor NR2B
      subunit mRNA is expressed at high levels. In the early postnatal period when
      active synaptogenesis takes place, both mRNAs became elevated and concentrated in
      the telencephalon and cerebellar granular layer, where NR2A and/or NR2B subunit
      mRNAs are abundantly expressed. Chapsyn-110 mRNA was, though at low levels, found
      over the mantle zone of embryonic brains, and the level was progressively
      increased in the telencephalon starting at perinatal stages. The spatial and
      temporal correlations in the brain in vivo suggest that the PSD-95/SAP90 protein 
      family can interact with NMDA receptor subunits to cluster them into channel
      complex at both synaptic and non-synaptic sites before, during and after
      synaptogenic stages.
FAU - Fukaya, M
AU  - Fukaya M
AD  - Department of Anatomy, Hokkaido University School of Medicine, Sapporo, Japan.
FAU - Ueda, H
AU  - Ueda H
FAU - Yamauchi, K
AU  - Yamauchi K
FAU - Inoue, Y
AU  - Inoue Y
FAU - Watanabe, M
AU  - Watanabe M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Antisense Elements (Genetics))
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, mouse)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SAP90-PSD95 Associated Proteins)
RN  - 0 (postsynaptic density proteins)
RN  - EC 2.7.4.4 (Nucleoside-Phosphate Kinase)
RN  - EC 2.7.4.8 (Dlgh2 protein, mouse)
RN  - EC 2.7.4.8 (Guanylate Kinases)
SB  - IM
MH  - Aging/metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Antisense Elements (Genetics)
MH  - Base Sequence
MH  - Brain/embryology/growth & development/*metabolism
MH  - Disks Large Homolog 4 Protein
MH  - Embryonic and Fetal Development
MH  - *Gene Expression Regulation, Developmental
MH  - Guanylate Kinases
MH  - Intracellular Signaling Peptides and Proteins
MH  - Membrane Proteins
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*genetics
MH  - Nucleoside-Phosphate Kinase/genetics
MH  - Organ Specificity
MH  - RNA, Messenger/genetics
MH  - SAP90-PSD95 Associated Proteins
MH  - Time Factors
MH  - *Transcription, Genetic
EDAT- 1999/04/22 00:00
MHDA- 1999/04/22 00:01
CRDT- 1999/04/22 00:00
PHST- 1999/04/22 00:00 [pubmed]
PHST- 1999/04/22 00:01 [medline]
PHST- 1999/04/22 00:00 [entrez]
AID - S0168-0102(98)00120-5 [pii]
AID - 10.1016/s0168-0102(98)00120-5 [doi]
PST - ppublish
SO  - Neurosci Res. 1999 Feb;33(2):111-8. doi: 10.1016/s0168-0102(98)00120-5.