PMID- 10211705
OWN - NLM
STAT- MEDLINE
DCOM- 19990607
LR  - 20191024
IS  - 0282-0080 (Print)
IS  - 0282-0080 (Linking)
VI  - 15
IP  - 10
DP  - 1998 Oct
TI  - Evidence for a site-specific fucosylation of N-linked oligosaccharide of
      immunoglobulin A1 from normal human serum.
PG  - 995-1000
AB  - Glycopeptides containing the N-linked oligosaccharide from human serum IgA1 were 
      analyzed by matrix-assisted laser desorption ionization time-of-flight mass
      spectrometry (MALDI-TOFMS). Two glycopeptides, GP1 and GP2, prepared from the
      endoproteinase Asp-N digest of the IgA1 heavy chain, were derived from the CH2
      domain (N-glycan site at Asn263) and the tailpiece portion (N-glycan site at
      Asn459), respectively. The structure of the attached sugar chain was deduced from
      the mass number of the glycopeptide and confirmed by a two-dimensional mapping
      technique for a pyridylaminated oligosaccharide. GP1 was composed of two major
      components having a fully galactosylated bianntena sugar chain with or without a 
      bisecting N-acetylglucosamine (GlcNAc) residue. On the other hand, the GP2
      fraction corresponded to the glycopeptides having a fully galactosylated and
      fucosylated bianntena sugar chain partly bearing a bisecting GlcNAc residue.
      Thus, the site-specific fucosylation of the N-linked oligosaccharide on the
      tailpiece of the alpha1 chain became evident for normal human serum IgA1.
FAU - Tanaka, A
AU  - Tanaka A
AD  - Analytical Research Laboratory, Asahi Chemical Industry Co., Ltd., Fuji,
      Shizuoka, Japan.
FAU - Iwase, H
AU  - Iwase H
FAU - Hiki, Y
AU  - Hiki Y
FAU - Kokubo, T
AU  - Kokubo T
FAU - Ishii-Karakasa, I
AU  - Ishii-Karakasa I
FAU - Toma, K
AU  - Toma K
FAU - Kobayashi, Y
AU  - Kobayashi Y
FAU - Hotta, K
AU  - Hotta K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glycoconj J
JT  - Glycoconjugate journal
JID - 8603310
RN  - 0 (Immunoglobulin A)
RN  - 0 (Oligosaccharides)
RN  - 28RYY2IV3F (Fucose)
SB  - IM
MH  - Amino Acid Sequence
MH  - Carbohydrate Sequence
MH  - Fucose/*metabolism
MH  - Humans
MH  - Immunoglobulin A/*blood/chemistry
MH  - Molecular Sequence Data
MH  - Oligosaccharides/chemistry/*metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 1999/04/22 00:00
MHDA- 1999/04/22 00:01
CRDT- 1999/04/22 00:00
PHST- 1999/04/22 00:00 [pubmed]
PHST- 1999/04/22 00:01 [medline]
PHST- 1999/04/22 00:00 [entrez]
AID - 10.1023/a:1006989910120 [doi]
PST - ppublish
SO  - Glycoconj J. 1998 Oct;15(10):995-1000. doi: 10.1023/a:1006989910120.