PMID- 10210332
OWN - NLM
STAT- MEDLINE
DCOM- 19990614
LR  - 20191024
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 27
IP  - 4
DP  - 1999 Apr
TI  - A homolog of the fungal nuclear migration gene nudC is involved in normal and
      malignant human hematopoiesis.
PG  - 742-50
AB  - The filamentous fungus Aspergillus nidulans nudC gene has an essential function
      in movement of nuclei following mitosis and is required for normal colony growth.
      Here, the molecular cloning and role in hematopoiesis of a human gene (designated
      HnudC) homologous to A. nidulans nudC is reported. The amino terminus of the
      larger human protein (HNUDC = 45 kDa) does not overlap with A. nidulans NUDC (22 
      kDa). However, NUDC and the C-terminal 94 amino acids of HNUDC are 67% identical.
      The C-terminal region of the HnudC gene fully complements the A. nidulans
      temperature-sensitive nudC3 mutation, suggesting that nudC has an essential
      function in cell growth that is conserved from filamentous fungi to humans. In
      initial studies, HNUDC levels were much higher in erythroid precursors compared
      to most other human tissues. Therefore, the potential role of HnudC in
      hematopoiesis was explored. In normal human bone marrow, HNUDC protein and mRNA
      are highly expressed in early myeloid and erythroid precursors and decline as
      these cells terminally differentiate. To determine whether hematopoietic growth
      factors induce HnudC expression, TF-1 cells were stimulated by
      granulocyte-macrophage colony-stimulating factor. This induced a significant
      increase in HNUDC protein and HnudC mRNA, suggesting that enhancement of HnudC
      expression in response to growth factor stimulation may be mediated at the
      transcription level. Furthermore, HNUDC was significantly enhanced in lysates of 
      bone marrow aspirates from patients with acute myelogenous and acute
      lymphoblastic leukemia compared to aspirates from normal controls, suggesting
      that HnudC is involved in malignant hematopoietic cell growth as well. These data
      demonstrate that HNUDC is highly expressed in normal and malignant human
      hematopoietic precursors and suggest it is of functional importance in the
      proliferation of these cells.
FAU - Miller, B A
AU  - Miller BA
AD  - Department of Pediatrics, The Pennsylvania State University College of Medicine, 
      The Milton S. Hershey Medical Center, Hershey 17033-0850, USA. bam11@psu.edu
FAU - Zhang, M Y
AU  - Zhang MY
FAU - Gocke, C D
AU  - Gocke CD
FAU - De Souza, C
AU  - De Souza C
FAU - Osmani, A H
AU  - Osmani AH
FAU - Lynch, C
AU  - Lynch C
FAU - Davies, J
AU  - Davies J
FAU - Bell, L
AU  - Bell L
FAU - Osmani, S A
AU  - Osmani SA
LA  - eng
SI  - GENBANK/AF130736
GR  - M01 RR 10732/RR/NCRR NIH HHS/United States
GR  - R01 DK 46778/DK/NIDDK NIH HHS/United States
GR  - R01 GM 42564/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (NUDC protein, Emericella nidulans)
RN  - 0 (NUDC protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proteins)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Antibody Specificity
MH  - Aspergillus nidulans/genetics
MH  - Bone Marrow Cells/cytology/drug effects/metabolism
MH  - Cell Cycle Proteins
MH  - Cell Differentiation/genetics
MH  - Cell Division/genetics
MH  - Cloning, Molecular
MH  - Fungal Proteins/*genetics/immunology
MH  - Gene Expression
MH  - Genes, Fungal/*genetics
MH  - Genetic Complementation Test
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Hematopoiesis/*genetics
MH  - Humans
MH  - Infant, Newborn
MH  - Leukemia, Myeloid, Acute/metabolism
MH  - Molecular Sequence Data
MH  - Nuclear Proteins
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/metabolism
MH  - Proteins/*genetics/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
EDAT- 1999/04/21 00:00
MHDA- 1999/04/21 00:01
CRDT- 1999/04/21 00:00
PHST- 1999/04/21 00:00 [pubmed]
PHST- 1999/04/21 00:01 [medline]
PHST- 1999/04/21 00:00 [entrez]
AID - S0301-472X(98)00074-5 [pii]
AID - 10.1016/s0301-472x(98)00074-5 [doi]
PST - ppublish
SO  - Exp Hematol. 1999 Apr;27(4):742-50. doi: 10.1016/s0301-472x(98)00074-5.