PMID- 10209272
OWN - NLM
STAT- MEDLINE
DCOM- 19990527
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1445
IP  - 1
DP  - 1999 Apr 14
TI  - Isolation and characterization of the gene encoding the mouse broad specificity
      lysosomal alpha-mannosidase1.
PG  - 177-83
AB  - A genomic clone encoding the mouse lysosomal alpha-mannosidases was isolated and 
      the gene was found to be encoded by 24 exons spanning approximately 14.5 kb of
      genomic DNA. The intron-exon boundaries were conserved between the mouse, human, 
      and bovine lysosomal alpha-mannosidase genes as well as being partially conserved
      in several other species. In order to define the promoter of the mouse
      mannosidase gene, >1 kb of DNA sequence was obtained upstream from the respective
      initiation codon. The transcription start site was identified by a 5'-RACE
      procedure and putative promoter elements were identified by expression of
      promoter/reporter constructs. Fluorescence in situ hybridization analysis using
      the mouse and human mannosidase genomic clones as probes, localized the mouse
      gene to chromosome 8, at band position 8C2, and the human gene to chromosome
      19p13.2, a region syntenic to the lysosomal mannosidase gene on mouse chromosome 
      8.
FAU - Gonzalez, D S
AU  - Gonzalez DS
AD  - Complex Carbohydrate Research Center and the Department of Biochemistry and
      Molecular Biology, Life Sciences Building, University of Georgia, Athens, GA
      30602, USA.
FAU - Kagawa, Y
AU  - Kagawa Y
FAU - Moremen, K W
AU  - Moremen KW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA, Complementary)
RN  - EC 3.2.1.- (Mannosidases)
RN  - EC 3.2.1.24 (alpha-Mannosidase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromosome Mapping
MH  - Conserved Sequence
MH  - DNA, Complementary/*chemistry/isolation & purification
MH  - Exons
MH  - Introns
MH  - Lysosomes/enzymology
MH  - Mannosidases/deficiency/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - alpha-Mannosidase
MH  - alpha-Mannosidosis/genetics
EDAT- 1999/04/21 00:00
MHDA- 1999/04/21 00:01
CRDT- 1999/04/21 00:00
PHST- 1999/04/21 00:00 [pubmed]
PHST- 1999/04/21 00:01 [medline]
PHST- 1999/04/21 00:00 [entrez]
AID - S0167-4781(99)00034-2 [pii]
AID - 10.1016/s0167-4781(99)00034-2 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Apr 14;1445(1):177-83. doi:
      10.1016/s0167-4781(99)00034-2.