PMID- 10209101
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20190728
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 9
IP  - 6
DP  - 1999 Mar 25
TI  - Association of the type 1 protein phosphatase PP1 with the A-kinase anchoring
      protein AKAP220.
PG  - 321-4
AB  - The cyclic AMP (cAMP)-dependent protein kinase (PKA) and the type 1 protein
      phosphatase (PP1) are broad-specificity signaling enzymes with opposing actions
      that catalyze changes in the phosphorylation state of cellular proteins.
      Subcellular targeting to the vicinity of preferred substrates is a means of
      restricting the specificity of each enzyme [1] [2]. Compartmentalization of the
      PKA holoenzyme is mediated through association of the regulatory subunits with
      A-kinase anchoring proteins (AKAPs), whereas a diverse family of
      phosphatase-targeting subunits directs the location of the PP1 catalytic subunit 
      (PP1c) [3] [4]. Here, we demonstrate that the PKA-anchoring protein, AKAP220,
      binds PP1c with a dissociation constant (KD) of 12.1 +/- 4 nM in vitro.
      Immunoprecipitation of PP1 from cell extracts resulted in a 10.4 +/- 3.8-fold
      enrichment of PKA activity. AKAP220 co-purified with PP1c by affinity
      chromatography on microcystin sepharos Immunocytochemical analysis demonstrated
      that the kinase, the phosphatase and the anchoring protein had distinct but
      overlapping staining patterns in rat hippocampal neurons. Collectively, these
      results provide the first evidence that AKAP220 is a multivalent anchoring
      protein that maintains a signaling scaffold of PP1 and the PKA holoenzyme.
FAU - Schillace, R V
AU  - Schillace RV
AD  - Howard Hughes Medical Institute, L-474 Vollum Institute, Oregon Health Sciences
      University, 3181 S.W. Sam Jackson Park Road, Portland, Oregon 97201-3098, USA.
FAU - Scott, J D
AU  - Scott JD
LA  - eng
GR  - DK48239/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (A Kinase Anchor Proteins)
RN  - 0 (AKAP11 protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microcystins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Peptides, Cyclic)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 77238-39-2 (microcystin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.16 (Protein Phosphatase 1)
SB  - IM
MH  - A Kinase Anchor Proteins
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Carrier Proteins
MH  - Cell Compartmentation
MH  - Cell Membrane/*chemistry/ultrastructure
MH  - Chromatography, Affinity
MH  - Cyclic AMP/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/*analysis
MH  - Enzyme Activation
MH  - Hippocampus/chemistry/*cytology
MH  - Humans
MH  - Macromolecular Substances
MH  - Membrane Proteins/*analysis
MH  - Microcystins
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*analysis
MH  - Neurites/chemistry/ultrastructure
MH  - Neurons/*chemistry/ultrastructure
MH  - Peptides, Cyclic/metabolism
MH  - Phosphoprotein Phosphatases/*analysis/genetics/metabolism
MH  - Phosphorylation
MH  - Protein Phosphatase 1
MH  - Protein Processing, Post-Translational
MH  - Proteins/*analysis
MH  - Rats
MH  - Recombinant Fusion Proteins/metabolism
MH  - Second Messenger Systems
EDAT- 1999/04/21 00:00
MHDA- 1999/04/21 00:01
CRDT- 1999/04/21 00:00
PHST- 1999/04/21 00:00 [pubmed]
PHST- 1999/04/21 00:01 [medline]
PHST- 1999/04/21 00:00 [entrez]
AID - S0960-9822(99)80141-9 [pii]
AID - 10.1016/s0960-9822(99)80141-9 [doi]
PST - ppublish
SO  - Curr Biol. 1999 Mar 25;9(6):321-4. doi: 10.1016/s0960-9822(99)80141-9.