PMID- 10209041
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 189
IP  - 8
DP  - 1999 Apr 19
TI  - GrpL, a Grb2-related adaptor protein, interacts with SLP-76 to regulate nuclear
      factor of activated T cell activation.
PG  - 1243-53
AB  - Propagation of signals from the T cell antigen receptor (TCR) involves a number
      of adaptor molecules. SH2 domain-containing protein 76 (SLP-76) interacts with
      the guanine nucleotide exchange factor Vav to activate the nuclear factor of
      activated cells (NF-AT), and its expression is required for normal T cell
      development. We report the cloning and characterization of a novel Grb2-like
      adaptor molecule designated as Grb2-related protein of the lymphoid system
      (GrpL). Expression of GrpL is restricted to hematopoietic tissues, and it is
      distinguished from Grb2 by having a proline-rich region. GrpL can be
      coimmunoprecipitated with SLP-76 but not with Sos1 or Sos2 from Jurkat cell
      lysates. In contrast, Grb2 can be coimmunoprecipitated with Sos1 and Sos2 but not
      with SLP-76. Moreover, tyrosine-phosphorylated LAT/pp36/38 in detergent lysates
      prepared from anti-CD3 stimulated T cells associated with Grb2 but not GrpL.
      These data reveal the presence of distinct complexes involving GrpL and Grb2 in T
      cells. A functional role of the GrpL-SLP-76 complex is suggested by the ability
      of GrpL to act alone or in concert with SLP-76 to augment NF-AT activation in
      Jurkat T cells.
FAU - Law, C L
AU  - Law CL
AD  - Department of Microbiology, University of Washington, Seattle, Washington 98195, 
      USA.
FAU - Ewings, M K
AU  - Ewings MK
FAU - Chaudhary, P M
AU  - Chaudhary PM
FAU - Solow, S A
AU  - Solow SA
FAU - Yun, T J
AU  - Yun TJ
FAU - Marshall, A J
AU  - Marshall AJ
FAU - Hood, L
AU  - Hood L
FAU - Clark, E A
AU  - Clark EA
LA  - eng
SI  - GENBANK/AF129476
SI  - GENBANK/AF129477
GR  - R01 GM037905/GM/NIGMS NIH HHS/United States
GR  - DE00859/DE/NIDCR NIH HHS/United States
GR  - GM37905/GM/NIGMS NIH HHS/United States
GR  - GM42508/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (CD3 Complex)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (GRAP2 protein, human)
RN  - 0 (Mona protein, mouse)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (SLP-76 signal Transducing adaptor proteins)
RN  - 0 (Transcription Factors)
RN  - 9DLQ4CIU6V (Proline)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - CD3 Complex/metabolism
MH  - Carrier Proteins/chemistry/metabolism
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/*metabolism
MH  - Gene Expression Regulation/genetics
MH  - Humans
MH  - Jurkat Cells
MH  - Mice
MH  - Molecular Sequence Data
MH  - NFATC Transcription Factors
MH  - *Nuclear Proteins
MH  - Phosphoproteins/*metabolism
MH  - Proline/genetics
MH  - RNA, Messenger/metabolism
MH  - Receptors, Antigen, T-Cell/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction/genetics
MH  - T-Lymphocytes/*metabolism
MH  - Transcription Factors/*metabolism
MH  - Transcriptional Activation/genetics
MH  - src Homology Domains/genetics
PMC - PMC2193019
EDAT- 1999/04/20 00:00
MHDA- 1999/04/20 00:01
CRDT- 1999/04/20 00:00
PHST- 1999/04/20 00:00 [pubmed]
PHST- 1999/04/20 00:01 [medline]
PHST- 1999/04/20 00:00 [entrez]
AID - 10.1084/jem.189.8.1243 [doi]
PST - ppublish
SO  - J Exp Med. 1999 Apr 19;189(8):1243-53. doi: 10.1084/jem.189.8.1243.