PMID- 10209034
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 145
IP  - 2
DP  - 1999 Apr 19
TI  - The integrin alpha9beta1 mediates adhesion to activated endothelial cells and
      transendothelial neutrophil migration through interaction with vascular cell
      adhesion molecule-1.
PG  - 413-20
AB  - The integrin alpha9beta1 has been shown to be widely expressed on smooth muscle
      and epithelial cells, and to mediate adhesion to the extracellular matrix
      proteins osteopontin and tenascin-C. We have found that the peptide sequence this
      integrin recognizes in tenascin-C is highly homologous to the sequence recognized
      by the closely related integrin alpha4beta1, in the inducible endothelial ligand,
      vascular cell adhesion mole-cule-1 (VCAM-1). We therefore sought to determine
      whether alpha9beta1 also recognizes VCAM-1, and whether any such interaction
      would be biologically significant. In this report, we demonstrate that
      alpha9beta1 mediates stable cell adhesion to recombinant VCAM-1 and to VCAM-1
      induced on human umbilical vein endothelial cells by tumor necrosis factor-alpha.
      Furthermore, we show that alpha9beta1 is highly and selectively expressed on
      neutrophils and is critical for neutrophil migration on VCAM-1 and tenascin-C.
      Finally, alpha9beta1 and alpha4 integrins contribute to neutrophil chemotaxis
      across activated endothelial monolayers. These observations suggest a possible
      role for alpha9beta1/VCAM-1 interactions in extravasation of neutrophils at sites
      of acute inflammation.
FAU - Taooka, Y
AU  - Taooka Y
AD  - Lung Biology Center, Center for Occupational and Environmental Health,
      Cardiovascular Research Institute and the Department of Medicine, University of
      California, San Francisco, California 94143, USA.
FAU - Chen, J
AU  - Chen J
FAU - Yednock, T
AU  - Yednock T
FAU - Sheppard, D
AU  - Sheppard D
LA  - eng
GR  - HLAI33259/HL/NHLBI NIH HHS/United States
GR  - P50 HL056385/HL/NHLBI NIH HHS/United States
GR  - HL47412/HL/NHLBI NIH HHS/United States
GR  - R37 HL053949/HL/NHLBI NIH HHS/United States
GR  - HL53949/HL/NHLBI NIH HHS/United States
GR  - R01 HL053949/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Antigens, CD)
RN  - 0 (Integrin alpha Chains)
RN  - 0 (Integrins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (integrin alpha 9 beta 1)
RN  - 0 (integrin alpha9)
RN  - 143198-26-9 (Integrin alpha4)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/physiology
MH  - CHO Cells
MH  - Cell Adhesion/*physiology
MH  - Cells, Cultured
MH  - *Chemotaxis, Leukocyte
MH  - Cricetinae
MH  - Endothelium, Vascular/*physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - *Integrin alpha Chains
MH  - Integrin alpha4
MH  - Integrins/genetics/*physiology
MH  - Neutrophils/*physiology
MH  - Recombinant Proteins/biosynthesis/pharmacology
MH  - Transfection
MH  - Umbilical Veins
MH  - Vascular Cell Adhesion Molecule-1/pharmacology/*physiology
PMC - PMC2133104
EDAT- 1999/04/20 00:00
MHDA- 1999/04/20 00:01
CRDT- 1999/04/20 00:00
PHST- 1999/04/20 00:00 [pubmed]
PHST- 1999/04/20 00:01 [medline]
PHST- 1999/04/20 00:00 [entrez]
AID - 10.1083/jcb.145.2.413 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Apr 19;145(2):413-20. doi: 10.1083/jcb.145.2.413.