PMID- 10209026
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20191210
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 145
IP  - 2
DP  - 1999 Apr 19
TI  - An essential role for katanin in severing microtubules in the neuron.
PG  - 305-15
AB  - Several lines of evidence suggest that microtubules are nucleated at the neuronal
      centrosome, and then released for transport into axons and dendrites. Here we
      sought to determine whether the microtubule-severing protein known as katanin
      mediates microtubule release from the neuronal centrosome. Immunomicroscopic
      analyses on cultured sympathetic neurons show that katanin is present at the
      centrosome, but is also widely distributed throughout the neuron. Microinjection 
      of an antibody that inactivates katanin results in a dramatic accumulation of
      microtubules at the centrosome, indicating that katanin is indeed required for
      microtubule release from the centrosome. However, the antibody also causes an
      inhibition of axon outgrowth that is more immediate than expected on this basis
      alone. It may be that katanin severs microtubules throughout the cell body to
      keep them sufficiently short to be efficiently transported into developing
      processes. Consistent with this idea, there were significantly fewer free ends of
      microtubules in the cell bodies of neurons that had been injected with the
      katanin antibody compared with controls. These results indicate that
      microtubule-severing by katanin is essential for releasing microtubules from the 
      neuronal centrosome, and also for regulating the length of the microtubules after
      their release.
FAU - Ahmad, F J
AU  - Ahmad FJ
AD  - Department of Anatomy, The University of Wisconsin Medical School, Madison,
      Wisconsin 53706, USA.
FAU - Yu, W
AU  - Yu W
FAU - McNally, F J
AU  - McNally FJ
FAU - Baas, P W
AU  - Baas PW
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 8064-90-2 (Trimethoprim, Sulfamethoxazole Drug Combination)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 5.6.1.1 (Katanin)
SB  - IM
MH  - Adenosine Triphosphatases/analysis/*metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Axons/physiology/ultrastructure
MH  - Cells, Cultured
MH  - Centrosome/ultrastructure
MH  - Ganglia, Sympathetic/cytology
MH  - Katanin
MH  - Microscopy, Electron
MH  - Microscopy, Immunoelectron
MH  - Microtubules/*ultrastructure
MH  - Neurons/enzymology/physiology/*ultrastructure
MH  - Rats
MH  - Trimethoprim, Sulfamethoxazole Drug Combination/analysis
PMC - PMC2133110
EDAT- 1999/04/20 00:00
MHDA- 1999/04/20 00:01
CRDT- 1999/04/20 00:00
PHST- 1999/04/20 00:00 [pubmed]
PHST- 1999/04/20 00:01 [medline]
PHST- 1999/04/20 00:00 [entrez]
AID - 10.1083/jcb.145.2.305 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Apr 19;145(2):305-15. doi: 10.1083/jcb.145.2.305.