PMID- 10209023
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 145
IP  - 2
DP  - 1999 Apr 19
TI  - Prefoldin-nascent chain complexes in the folding of cytoskeletal proteins.
PG  - 265-77
AB  - In vitro transcription/translation of actin cDNA and analysis of the translation 
      products by native-PAGE was used to study the maturation pathway of actin. During
      the course of actin synthesis, several distinct actin-containing species were
      observed and the composition of each determined by immunological procedures.
      After synthesis of the first approximately 145 amino acids, the nascent
      ribosome-associated actin chain binds to the recently identified heteromeric
      chaperone protein, prefoldin (PFD). PFD remains bound to the relatively unfolded 
      actin polypeptide until its posttranslational delivery to cytosolic chaperonin
      (CCT). We show that alpha- and beta-tubulin follow a similar maturation pathway, 
      but to date find no evidence for an interaction between PFD and several
      noncytoskeletal proteins. We conclude that PFD functions by selectively targeting
      nascent actin and tubulin chains pending their transfer to CCT for final folding 
      and/or assembly.
FAU - Hansen, W J
AU  - Hansen WJ
AD  - Surgical Research Laboratory, San Francisco General Hospital, Departments of
      Surgery, Medicine, and Physiology, University of California, San Francisco,
      California 94143, USA. hanswb@itsa.ucsf.edu
FAU - Cowan, N J
AU  - Cowan NJ
FAU - Welch, W J
AU  - Welch WJ
LA  - eng
GR  - R01 GM033551/GM/NIGMS NIH HHS/United States
GR  - GM33551/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Actins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fungal Proteins)
RN  - 0 (Molecular Chaperones)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tubulin)
RN  - 0 (prefoldin)
SB  - IM
MH  - Actins/*biosynthesis/*chemistry/genetics/isolation & purification
MH  - Animals
MH  - Chickens
MH  - Cytoskeletal Proteins/*biosynthesis/*chemistry
MH  - DNA, Complementary
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fungal Proteins/metabolism
MH  - Kinetics
MH  - Models, Molecular
MH  - Molecular Chaperones/*metabolism
MH  - Protein Binding
MH  - Protein Biosynthesis
MH  - Protein Denaturation
MH  - Protein Folding
MH  - Protein Structure, Secondary
MH  - RNA, Messenger/genetics/metabolism
MH  - Rabbits
MH  - Reticulocytes/metabolism
MH  - Ribosomes/*metabolism
MH  - Transcription, Genetic
MH  - Tubulin/biosynthesis/chemistry
PMC - PMC2133115
EDAT- 1999/04/20 00:00
MHDA- 1999/04/20 00:01
CRDT- 1999/04/20 00:00
PHST- 1999/04/20 00:00 [pubmed]
PHST- 1999/04/20 00:01 [medline]
PHST- 1999/04/20 00:00 [entrez]
AID - 10.1083/jcb.145.2.265 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Apr 19;145(2):265-77. doi: 10.1083/jcb.145.2.265.