PMID- 10209021
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 145
IP  - 2
DP  - 1999 Apr 19
TI  - RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at 
      the nuclear pore complex through multiple domains.
PG  - 237-54
AB  - Gle2p is implicated in nuclear export of poly(A)+ RNA and nuclear pore complex
      (NPC) structure and distribution in Saccharomyces cerevisiae. Gle2p is anchored
      at the nuclear envelope (NE) via a short Gle2p-binding motif within Nup116p
      called GLEBS. The molecular mechanism by which Gle2p and the Gle2p-Nup116p
      interaction function in mRNA export is unknown. Here we show that RAE1, the
      mammalian homologue of Gle2p, binds to a GLEBS-like NUP98 motif at the NPC
      through multiple domains that include WD-repeats and a COOH-terminal
      non-WD-repeat extension. This interaction is direct, as evidenced by in vitro
      binding studies and chemical cross-linking. Microinjection experiments performed 
      in Xenopus laevis oocytes demonstrate that RAE1 shuttles between the nucleus and 
      the cytoplasm and is exported from the nucleus in a temperature-dependent and
      RanGTP-independent manner. Docking of RAE1 to the NE is highly dependent on new
      mRNA synthesis. Overexpression of the GLEBS-like motif also inhibits NE binding
      of RAE1 and induces nuclear accumulation of poly(A)+ RNA. Both effects are
      abrogated either by the introduction of point mutations in the GLEBS-like motif
      or by overexpression of RAE1, indicating a direct role for RAE1 and the
      NUP98-RAE1 interaction in mRNA export. Together, our data suggest that RAE1 is a 
      shuttling transport factor that directly contributes to nuclear export of mRNAs
      through its ability to anchor to a specific NUP98 motif at the NPC.
FAU - Pritchard, C E
AU  - Pritchard CE
AD  - Department of Genetics, St. Jude Children's Research Hospital, Memphis, Tennessee
      38105, USA.
FAU - Fornerod, M
AU  - Fornerod M
FAU - Kasper, L H
AU  - Kasper LH
FAU - van Deursen, J M
AU  - van Deursen JM
LA  - eng
GR  - P30 CA021765/CA/NCI NIH HHS/United States
GR  - R01 CA077262/CA/NCI NIH HHS/United States
GR  - CA-21765/CA/NCI NIH HHS/United States
GR  - R01 CA77262-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Fungal Proteins)
RN  - 0 (GLE2 protein, S cerevisiae)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nuclear Matrix-Associated Proteins)
RN  - 0 (Nuclear Pore Complex Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleocytoplasmic Transport Proteins)
RN  - 0 (RAE1 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Rae1 protein, mouse)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Schizosaccharomyces pombe Proteins)
RN  - 0 (nuclear pore complex protein 98)
RN  - 0 (rae1 protein, S pombe)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Cell Line
MH  - Cricetinae
MH  - Cross-Linking Reagents
MH  - Dactinomycin/pharmacology
MH  - Female
MH  - Fungal Proteins/*metabolism
MH  - Humans
MH  - Membrane Proteins/chemistry/genetics/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nuclear Envelope/*metabolism/physiology
MH  - *Nuclear Matrix-Associated Proteins
MH  - *Nuclear Pore Complex Proteins
MH  - Nuclear Proteins/chemistry/genetics/*metabolism
MH  - *Nucleocytoplasmic Transport Proteins
MH  - Oocytes/physiology
MH  - RNA Polymerase II/metabolism
MH  - RNA, Messenger/*metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Repetitive Sequences, Amino Acid
MH  - Saccharomyces cerevisiae/*genetics/*metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - *Schizosaccharomyces pombe Proteins
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Transfection
MH  - Xenopus laevis
PMC - PMC2133102
EDAT- 1999/04/20 00:00
MHDA- 1999/04/20 00:01
CRDT- 1999/04/20 00:00
PHST- 1999/04/20 00:00 [pubmed]
PHST- 1999/04/20 00:01 [medline]
PHST- 1999/04/20 00:00 [entrez]
AID - 10.1083/jcb.145.2.237 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Apr 19;145(2):237-54. doi: 10.1083/jcb.145.2.237.