PMID- 10207176
OWN - NLM
STAT- MEDLINE
DCOM- 19990607
LR  - 20190513
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 9
IP  - 5
DP  - 1999 May
TI  - Recombinant human interleukin-12 is the second example of a C-mannosylated
      protein.
PG  - 435-41
AB  - The beta-chain of human interleukin 12 (IL-12) contains at position 319-322, the 
      sequence Trp-x-x-Trp. In human RNase 2 this is the recognition motif for a new,
      recently discovered posttranslational modification, i.e., the C-glycosidic
      attachment of a mannosyl residue to the side chain of tryptophan. Analysis of
      C-terminal peptides of recombinant IL-12 (rHuIL-12) by mass spectrometry and NMR 
      spectroscopy revealed that Trp-319beta is (partially) C-mannosylated. This
      finding was extended by in vitro mannosylation experiments, using a synthetic
      peptide derived from the same region of the protein as an acceptor. Furthermore, 
      human B-lymphoblastoid cells, which secrete IL-12, were found to contain an
      enzyme that carries out the C-mannosylation reaction. This shows that
      nonrecombinant IL-12 is potentially C-mannosylated as well. This is only the
      second report on a C-mannosylated protein. However, the occurrence of the
      C-mannosyltransferase activity in a variety of cells and tissues, and the
      presence of the recognition motif in many proteins indicate that more
      C-mannosylated proteins may be found.
FAU - Doucey, M A
AU  - Doucey MA
AD  - Friedrich Miescher-Institut, P.O. Box 2543, CH-4002 Basel, Switzerland.
FAU - Hess, D
AU  - Hess D
FAU - Blommers, M J
AU  - Blommers MJ
FAU - Hofsteenge, J
AU  - Hofsteenge J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - 187348-17-0 (Interleukin-12)
RN  - EC 2.4.1.- (Mannosyltransferases)
RN  - PHA4727WTP (Mannose)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - B-Lymphocytes/metabolism
MH  - CHO Cells
MH  - Cricetinae
MH  - Glycosylation
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-12/*chemistry/metabolism
MH  - Magnetic Resonance Spectroscopy
MH  - Mannose/*chemistry/metabolism
MH  - Mannosyltransferases/metabolism
MH  - Peptide Fragments/chemistry/metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Substrate Specificity
EDAT- 1999/04/20 00:00
MHDA- 1999/04/20 00:01
CRDT- 1999/04/20 00:00
PHST- 1999/04/20 00:00 [pubmed]
PHST- 1999/04/20 00:01 [medline]
PHST- 1999/04/20 00:00 [entrez]
AID - cwc041 [pii]
AID - 10.1093/glycob/9.5.435 [doi]
PST - ppublish
SO  - Glycobiology. 1999 May;9(5):435-41. doi: 10.1093/glycob/9.5.435.