PMID- 10207106
OWN - NLM
STAT- MEDLINE
DCOM- 19990518
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 5
DP  - 1999 May
TI  - Isolation of a mammalian homologue of a fission yeast differentiation regulator.
PG  - 3829-41
AB  - In the fission yeast Schizosaccharomyces pombe the nrd1(+) gene encoding an RNA
      binding protein negatively regulates the onset of differentiation. Its biological
      role is to block differentiation by repressing a subset of the Ste11-regulated
      genes essential for conjugation and meiosis until the cells reach a critical
      level of nutrient starvation. By using the phenotypic suppression of the S. pombe
      temperature-sensitive pat1 mutant that commits lethal haploid meiosis at the
      restrictive temperature, we have cloned ROD1, a functional homologue of nrd1(+), 
      from rat and human cDNA libraries. Like nrd1(+), ROD1 encodes a protein with four
      repeats of typical RNA binding domains, though its amino acid homology to Nrd1 is
      limited. When expressed in the fission yeast, ROD1 behaves in a way that is
      functionally similar to nrd1(+), being able to repress Ste11-regulated genes and 
      to inhibit conjugation upon overexpression. ROD1 is predominantly expressed in
      hematopoietic cells or organs of adult and embryonic rat. Like nrd1(+) for
      fission yeast differentiation, overexpressed ROD1 effectively blocks both
      12-O-tetradecanoyl phorbol-13-acetate-induced megakaryocytic and sodium
      butyrate-induced erythroid differentiation of the K562 human leukemia cells
      without affecting their proliferative ability. These results suggest a role for
      ROD1 in differentiation control in mammalian cells. We discuss the possibility
      that a differentiation control system found in the fission yeast might well be
      conserved in more complex organisms, including mammals.
FAU - Yamamoto, H
AU  - Yamamoto H
AD  - Department of Biochemistry and Molecular Biology, Graduate School of Medicine,
      The University of Tokyo, Bunkyo-Ku, Tokyo 113-0033, Japan.
FAU - Tsukahara, K
AU  - Tsukahara K
FAU - Kanaoka, Y
AU  - Kanaoka Y
FAU - Jinno, S
AU  - Jinno S
FAU - Okayama, H
AU  - Okayama H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Butyrates)
RN  - 0 (Fungal Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (PTBP3 protein, human)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Schizosaccharomyces pombe Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (nrd1 protein, S pombe)
RN  - 139076-35-0 (Polypyrimidine Tract-Binding Protein)
RN  - 145139-18-0 (ste11 protein, S pombe)
RN  - 25191-14-4 (Poly G)
RN  - 27416-86-0 (Poly U)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Butyrates/pharmacology
MH  - Cell Differentiation/*genetics
MH  - Cloning, Molecular
MH  - Flow Cytometry
MH  - Fungal Proteins/chemistry/genetics
MH  - Gene Expression Regulation/genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/chemistry/genetics
MH  - Poly G/metabolism
MH  - Poly U/metabolism
MH  - Polypyrimidine Tract-Binding Protein
MH  - RNA-Binding Proteins/chemistry/*genetics
MH  - Rats
MH  - Ribonucleoproteins/chemistry
MH  - Schizosaccharomyces/genetics
MH  - *Schizosaccharomyces pombe Proteins
MH  - Sequence Homology, Amino Acid
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transcription Factors/genetics
MH  - Tumor Cells, Cultured
PMC - PMC84229
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - 10.1128/mcb.19.5.3829 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 May;19(5):3829-41. doi: 10.1128/mcb.19.5.3829.