PMID- 10207080
OWN - NLM
STAT- MEDLINE
DCOM- 19990518
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 5
DP  - 1999 May
TI  - FKLF, a novel Kruppel-like factor that activates human embryonic and fetal
      beta-like globin genes.
PG  - 3571-9
AB  - A cDNA encoding a novel Kruppel-type zinc finger protein, FKLF, was cloned from
      fetal globin-expressing human fetal erythroid cells. The deduced polypeptide
      sequence composed of 512 amino acids revealed that, like Sp1 and EKLF, FKLF has
      three contiguous zinc fingers at the near carboxyl-terminal end. A long
      amino-terminal domain is characterized by the presence of two acidic and two
      proline-rich regions. Reverse transcription (RT)-PCR assays using various cell
      lines demonstrated that the FKLF mRNA is expressed predominantly in erythroid
      cells. FKLF message is detectable by RT-PCR in fetal liver but not in adult bone 
      marrow cells. As predicted from its structural features, FKLF is a
      transcriptional activator. In luciferase assays FKLF activated the gamma- and
      epsilon-globin gene promoters, and, to a much lower degree, the beta-globin
      promoter. Studies of HS2-gamma gene reporter constructs carrying CACCC box
      deletions revealed that the CACCC box sequence of the gamma gene promoter
      mediates the activation of the gamma gene by FKLF. Other erythroid promoters
      (GATA-1, glycophorin B, ferrochelatase, porphobilinogen deaminase, and
      5-aminolevulinate synthase) containing CACCC elements or GC-rich potential
      Sp1-binding sites were activated minimally, if at all, by FKLF, indicating that
      FKLF is not a general activator of genes carrying the CACCC motifs. Transfection 
      of K562 cells with FKLF cDNA enhanced the expression of the endogenous epsilon-
      and gamma-globin genes, suggesting an in vivo role of FKLF in fetal and embryonic
      globin gene expression. Our results indicate that the protein potentially encoded
      by the FKLF cDNA acts as a transcriptional activator of embryonic and fetal
      beta-like globin genes.
FAU - Asano, H
AU  - Asano H
AD  - Division of Medical Genetics, University of Washington, Seattle, Washington, USA.
FAU - Li, X S
AU  - Li XS
FAU - Stamatoyannopoulos, G
AU  - Stamatoyannopoulos G
LA  - eng
SI  - GENBANK/AF272830
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KLF11 protein, human)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 9004-22-2 (Globins)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Cell Cycle Proteins
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/*genetics
MH  - Gene Expression Regulation/*genetics
MH  - Gene Expression Regulation, Developmental/genetics
MH  - Genes, Reporter
MH  - Globins/*genetics
MH  - Humans
MH  - Kruppel-Like Transcription Factors
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic/genetics
MH  - RNA, Messenger/metabolism
MH  - *Repressor Proteins
MH  - Sequence Analysis, DNA
MH  - Trans-Activators/chemistry/*genetics
MH  - Transcription Factors/*genetics
MH  - Transfection
MH  - Zinc Fingers/genetics
PMC - PMC84149
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - 10.1128/mcb.19.5.3571 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 May;19(5):3571-9. doi: 10.1128/mcb.19.5.3571.