PMID- 10207077
OWN - NLM
STAT- MEDLINE
DCOM- 19990518
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 5
DP  - 1999 May
TI  - The carboxyl terminus of RNA helicase A contains a bidirectional nuclear
      transport domain.
PG  - 3540-50
AB  - Human RNA helicase A was recently identified to be a shuttle protein which
      interacts with the constitutive transport element (CTE) of type D retroviruses.
      Here we show that a domain of 110 amino acids at the carboxyl terminus of
      helicase A is both necessary and sufficient for nuclear localization as well as
      rapid nuclear export of glutathione S-transferase fusion proteins. The import and
      export activities of this domain overlap but are separable by point mutations.
      This bidirectional nuclear transport domain (NTD) has no obvious sequence
      homology to previously identified nuclear import or export signals. However, the 
      Ran-dependent nuclear import of NTD was efficiently competed by excess amounts of
      the nuclear localization signal (NLS) peptide from simian virus 40 large T
      antigen, suggesting that import is mediated by the classical NLS pathway. The
      nuclear export pathway accessed by NTD is insensitive to leptomycin B and thus is
      distinct from the leucine-rich nuclear export signal pathway mediated by CRM1.
FAU - Tang, H
AU  - Tang H
AD  - Department of Biology and Department of Medicine, University of California, San
      Diego, CA, USA.
FAU - McDonald, D
AU  - McDonald D
FAU - Middlesworth, T
AU  - Middlesworth T
FAU - Hope, T J
AU  - Hope TJ
FAU - Wong-Staal, F
AU  - Wong-Staal F
LA  - eng
GR  - AI35477/AI/NIAID NIH HHS/United States
GR  - GM56089/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Antigens, Polyomavirus Transforming)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.6.4.13 (RNA Helicases)
RN  - EC 3.6.5.2 (ran GTP-Binding Protein)
RN  - Y031I2N1EO (leptomycin B)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Polyomavirus Transforming/genetics
MH  - Biological Transport/genetics
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Fatty Acids, Unsaturated/pharmacology
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Luminescent Proteins/genetics
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Nuclear Localization Signals/*genetics
MH  - Nuclear Proteins/genetics
MH  - Peptide Fragments/genetics/pharmacology
MH  - Protein Sorting Signals/genetics
MH  - RNA Helicases/chemistry/*genetics
MH  - Recombinant Fusion Proteins/genetics
MH  - Sequence Deletion/genetics
MH  - ran GTP-Binding Protein
PMC - PMC84146
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - 10.1128/MCB.19.5.3540 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 May;19(5):3540-50. doi: 10.1128/MCB.19.5.3540.