PMID- 10207075
OWN - NLM
STAT- MEDLINE
DCOM- 19990518
LR  - 20191210
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 5
DP  - 1999 May
TI  - Rec8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of
      the Rad21p family conserved from fission yeast to humans.
PG  - 3515-28
AB  - Our work and that of others defined mitosis-specific (Rad21 subfamily) and
      meiosis-specific (Rec8 subfamily) proteins involved in sister chromatid cohesion 
      in several eukaryotes, including humans. Mutation of the fission yeast
      Schizosaccharomyces pombe rec8 gene was previously shown to confer a number of
      meiotic phenotypes, including strong reduction of recombination frequencies in
      the central region of chromosome III, absence of linear element polymerization,
      reduced pairing of homologous chromosomes, reduced sister chromatid cohesion,
      aberrant chromosome segregation, defects in spore formation, and reduced spore
      viability. Here we extend the description of recombination reduction to the
      central regions of chromosomes I and II. We show at the protein level that
      expression of rec8 is meiosis specific and that Rec8p localizes to approximately 
      100 foci per prophase nucleus. Rec8p was present in an unphosphorylated form
      early in meiotic prophase but was phosphorylated prior to meiosis I, as
      demonstrated by analysis of the mei4 mutant blocked before meiosis I. Evidence
      for the persistence of Rec8p beyond meiosis I was obtained by analysis of the
      mutant mes1 blocked before meiosis II. A human gene, which we designate hrec8,
      showed significant primary sequence similarity to rec8 and was mapped to
      chromosome 14. High mRNA expression of mouse and human rec8 genes was found only 
      in germ line cells, specifically in testes and, interestingly, in spermatids.
      hrec8 was also expressed at a low level in the thymus. Sequence similarity and
      testis-specific expression indicate evolutionarily conserved functions of Rec8p
      in meiosis. Possible roles of Rec8p in the integration of different meiotic
      events are discussed.
FAU - Parisi, S
AU  - Parisi S
AD  - Institute of General Microbiology, University of Bern, 3012 Bern, Switzerland.
FAU - McKay, M J
AU  - McKay MJ
FAU - Molnar, M
AU  - Molnar M
FAU - Thompson, M A
AU  - Thompson MA
FAU - van der Spek, P J
AU  - van der Spek PJ
FAU - van Drunen-Schoenmaker, E
AU  - van Drunen-Schoenmaker E
FAU - Kanaar, R
AU  - Kanaar R
FAU - Lehmann, E
AU  - Lehmann E
FAU - Hoeijmakers, J H
AU  - Hoeijmakers JH
FAU - Kohli, J
AU  - Kohli J
LA  - eng
SI  - GENBANK/AF006264
SI  - GENBANK/AJ223299
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RAD21 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Rad21 protein, S pombe)
RN  - 0 (Rad21 protein, mouse)
RN  - 0 (Schizosaccharomyces pombe Proteins)
RN  - 148813-46-1 (REC8 protein, S pombe)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Cell Cycle Proteins
MH  - Chromatids/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes/genetics
MH  - Chromosomes, Human, Pair 14
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins
MH  - Eukaryotic Cells
MH  - Evolution, Molecular
MH  - Fungal Proteins/*genetics/metabolism
MH  - Gene Expression/genetics
MH  - Genetic Complementation Test
MH  - Germ Cells/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Meiosis/*genetics
MH  - Molecular Sequence Data
MH  - Mutation/genetics
MH  - Nuclear Proteins/*genetics
MH  - Phosphoproteins/*genetics
MH  - Phosphorylation
MH  - Phylogeny
MH  - RNA, Messenger/metabolism
MH  - Recombination, Genetic/*genetics
MH  - Saccharomyces/genetics
MH  - *Schizosaccharomyces pombe Proteins
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
PMC - PMC84144
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - 10.1128/mcb.19.5.3515 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 May;19(5):3515-28. doi: 10.1128/mcb.19.5.3515.