PMID- 10207050
OWN - NLM
STAT- MEDLINE
DCOM- 19990518
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 5
DP  - 1999 May
TI  - Cumulative effect of phosphorylation of pRB on regulation of E2F activity.
PG  - 3246-56
AB  - The product of the retinoblastoma susceptibility gene, pRB, is a nuclear
      phosphoprotein that controls cell growth by binding to and suppressing the
      activities of transcription factors such as the E2F family. Transactivation
      activity is inhibited when E2F is bound to hypophosphorylated pRB and released
      when pRB is phosphorylated by cyclin-dependent kinases (CDKs). To determine which
      of 16 potential CDK phosphorylation sites regulated the pRB-E2F interaction,
      mutant pRB proteins produced by site-directed mutagenesis were tested for the
      ability to suppress E2F-mediated transcription in a reporter chloramphenicol
      acetyltransferase assay. Surprisingly, no one CDK site regulated the interaction 
      of pRB with E2F when E2F was bound to DNA. Instead, disruption of transcriptional
      repression resulted from accumulation of phosphate groups on the RB molecule.
FAU - Brown, V D
AU  - Brown VD
AD  - Department of Molecular and Medical Genetics, University of Toronto, Toronto,
      Ontario M5G 1X8, Canada.
FAU - Phillips, R A
AU  - Phillips RA
FAU - Gallie, B L
AU  - Gallie BL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Arid4a protein, mouse)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin E)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (E2F Transcription Factors)
RN  - 0 (Phosphoproteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Retinoblastoma-Binding Protein 1)
RN  - 0 (Transcription Factor DP1)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.22 (CDC2-CDC28 Kinases)
RN  - EC 2.7.11.22 (Cdk2 protein, mouse)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 2)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - Animals
MH  - Binding Sites/genetics
MH  - *CDC2-CDC28 Kinases
MH  - COS Cells
MH  - *Carrier Proteins
MH  - *Cell Cycle Proteins
MH  - Cyclin E/metabolism
MH  - Cyclin-Dependent Kinase 2
MH  - Cyclin-Dependent Kinases/metabolism
MH  - DNA-Binding Proteins/genetics
MH  - E2F Transcription Factors
MH  - Gene Expression Regulation/genetics
MH  - Genes, Reporter/genetics
MH  - Mice
MH  - Mutagenesis, Site-Directed/genetics
MH  - Mutation/genetics
MH  - Phosphoproteins/genetics/metabolism
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/metabolism
MH  - Repressor Proteins/genetics
MH  - Retinoblastoma Protein/*genetics/metabolism
MH  - Retinoblastoma-Binding Protein 1
MH  - Transcription Factor DP1
MH  - Transcription Factors/*genetics/metabolism
MH  - Tumor Cells, Cultured
PMC - PMC84119
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - 10.1128/mcb.19.5.3246 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 May;19(5):3246-56. doi: 10.1128/mcb.19.5.3246.