PMID- 10207047
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 17
DP  - 1999 Apr 23
TI  - The src homology domain 2-containing inositol phosphatase SHIP forms a ternary
      complex with Shc and Grb2 in antigen receptor-stimulated B lymphocytes.
PG  - 12183-91
AB  - The inositol phosphatase SHIP has been implicated in signaling events downstream 
      of a variety of receptors and is thought to play an inhibitory role in stimulated
      B cells. We and others have reported that SHIP is rapidly tyrosine phosphorylated
      upon B cell antigen receptor (BCR) cross-linking and forms a complex with the
      adapter protein Shc. Here, we report that cross-linking of the BCR induces
      association between Grb2 and SHIP as well as association between Shc and SHIP. We
      made use of a Grb2-deficient B cell line to demonstrate both in vitro and in vivo
      that Grb2 expression is required for the efficient association between Shc and
      SHIP. The results indicate that SHIP, Shc, and Grb2 form a ternary complex in
      stimulated B cells, with Grb2 stabilizing the interaction between Shc and SHIP.
      The interactions between Shc, Grb2, and SHIP are therefore analogous to the
      interactions between Shc, Grb2, and SOS. Shc and Grb2 may help to localize SHIP
      to the cell membrane, regulating SHIP's inhibitory function following BCR
      stimulation.
FAU - Harmer, S L
AU  - Harmer SL
AD  - G. W. Hooper Foundation and the Departments of Biochemistry and Biophysics and
      Microbiology and Immunology, University of California, San Francisco, California 
      94143, USA.
FAU - DeFranco, A L
AU  - DeFranco AL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (GRB2 Adaptor Protein)
RN  - 0 (Grb2 protein, mouse)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
RN  - EC 3.1.3.86 (INPPL1 protein, human)
RN  - EC 3.1.3.86 (Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - B-Lymphocytes/*metabolism
MH  - Binding Sites
MH  - Cell Line
MH  - GRB2 Adaptor Protein
MH  - Lymphocyte Activation
MH  - Mice
MH  - Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases
MH  - Phosphoric Monoester Hydrolases/*metabolism
MH  - Proteins/*metabolism
MH  - Receptors, Antigen, B-Cell/*metabolism
MH  - *src Homology Domains
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - 10.1074/jbc.274.17.12183 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 23;274(17):12183-91. doi: 10.1074/jbc.274.17.12183.