PMID- 10207026
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20210402
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 17
DP  - 1999 Apr 23
TI  - Identification of the activating and conjugating enzymes of the NEDD8 conjugation
      pathway.
PG  - 12036-42
AB  - NEDD8 is a ubiquitin-like molecule that can be covalently conjugated to a limited
      number of cellular proteins, such as Cdc53/cullin. We have previously reported
      that the C terminus of NEDD8 is efficiently processed to expose Gly-76, which is 
      required for conjugation to target proteins. A combination of data base searches 
      and polymerase chain reaction cloning was used to identify a cDNA encoding human 
      UBA3, which is 38% identical to the yeast homologue, 22% identical to human UBA2,
      and 19% identical to the C-terminal region of human UBE1. The human UBA3 gene is 
      located on chromosome 3p13 and gave rise to a 2.2-kilobase pair transcript that
      was detected in all tissues. Human UBA3 could be precipitated with glutathione
      S-transferase (GST)-NEDD8, but not with GST-ubiquitin or GST-sentrin-1. Moreover,
      human UBA3 could form a beta-mercaptoethanol-sensitive conjugate with NEDD8 in
      the presence of APP-BP1, a protein with sequence homology to the N-terminal half 
      of ubiquitin-activating enzyme. We have also cloned human UBC12 and demonstrated 
      that it could form a thiol ester linkage with NEDD8 in the presence of the
      activating enzyme complex. Identification of the activating and conjugating
      enzymes of the NEDD8 conjugation pathway should allow for a more detailed study
      of the role of NEDD8 modification in health and disease.
FAU - Gong, L
AU  - Gong L
AD  - Research Center for Cardiovascular Diseases, Institute of Molecular Medicine for 
      the Prevention of Human Diseases, Department of Internal Medicine, University of 
      Texas-Houston Health Science Center, Houston, TX, USA.
FAU - Yeh, E T
AU  - Yeh ET
LA  - eng
SI  - GENBANK/AF046024
GR  - HL-45851/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (NEDD8 Protein)
RN  - 0 (NEDD8 protein, human)
RN  - 0 (Nedd8 protein, mouse)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (UBA3 protein, S cerevisiae)
RN  - 0 (Ube1c protein, mouse)
RN  - 0 (Ubiquitins)
RN  - EC 6.- (Ligases)
RN  - EC 6.2.1.45 (Ubiquitin-Activating Enzymes)
RN  - EC 6.3.2.- (NAE protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 3
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Humans
MH  - Ligases/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - NEDD8 Protein
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Recombinant Proteins/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Homology, Amino Acid
MH  - Ubiquitin-Activating Enzymes
MH  - Ubiquitins/genetics/*metabolism
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - 10.1074/jbc.274.17.12036 [doi]
AID - S0021-9258(19)73527-3 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 23;274(17):12036-42. doi: 10.1074/jbc.274.17.12036.