PMID- 10206991
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 17
DP  - 1999 Apr 23
TI  - Cloning of a new mouse two-P domain channel subunit and a human homologue with a 
      unique pore structure.
PG  - 11751-60
AB  - Mouse KCNK6 is a new subunit belonging to the TWIK channel family. This 335-amino
      acid polypeptide has four transmembrane segments, two pore-forming domains, and a
      Ca2+-binding EF-hand motif. Expression of KCNK6 transcripts is principally
      observed in eyes, lung, stomach and embryo. In the eyes, immunohistochemistry
      reveals protein expression only in some of the retina neurons. Although KCNK6 is 
      able to dimerize as other functional two-P domain K+ channels when it is
      expressed in COS-7 cells, it remains in the endoplasmic reticulum and is unable
      to generate ionic channel activity. Deletions, mutations, and chimera
      constructions suggest that KCNK6 is not an intracellular channel but rather a
      subunit that needs to associate with a partner, which remains to be discovered,
      in order to reach the plasma membrane. A closely related human KCNK7-A subunit
      has been cloned. KCNK7 displays an intriguing GLE sequence in its filter region
      instead of the G(Y/F/L)G sequence, which is considered to be the K+ channel
      signature. This subunit is alternatively spliced and gives rise to the shorter
      forms KCNK7-B and -C. None of the KCNK7 structures can generate channel activity 
      by itself. The KCNK7 gene is situated on chromosome 11, in the q13 region, where 
      several candidate diseases have been identified.
FAU - Salinas, M
AU  - Salinas M
AD  - Institut de Pharmacologie Moleculaire et Cellulaire, CNRS, 660 route des
      Lucioles, Sophia Antipolis, 06560 Valbonne, France.
FAU - Reyes, R
AU  - Reyes R
FAU - Lesage, F
AU  - Lesage F
FAU - Fosset, M
AU  - Fosset M
FAU - Heurteaux, C
AU  - Heurteaux C
FAU - Romey, G
AU  - Romey G
FAU - Lazdunski, M
AU  - Lazdunski M
LA  - eng
SI  - GENBANK/AF110521
SI  - GENBANK/AF110522
SI  - GENBANK/AF110523
SI  - GENBANK/AF110524
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (KCNK1 protein, human)
RN  - 0 (Kcnk1 protein, mouse)
RN  - 0 (Potassium Channels)
RN  - 0 (Potassium Channels, Tandem Pore Domain)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 11
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Potassium Channels/chemistry/*genetics
MH  - *Potassium Channels, Tandem Pore Domain
MH  - RNA Splicing
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Xenopus
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - 10.1074/jbc.274.17.11751 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 23;274(17):11751-60. doi: 10.1074/jbc.274.17.11751.