PMID- 10206961
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 17
DP  - 1999 Apr 23
TI  - An APAF-1.cytochrome c multimeric complex is a functional apoptosome that
      activates procaspase-9.
PG  - 11549-56
AB  - We report here the reconstitution of the de novo procaspase-9 activation pathway 
      using highly purified cytochrome c, recombinant APAF-1, and recombinant
      procaspase-9. APAF-1 binds and hydrolyzes ATP or dATP to ADP or dADP,
      respectively. The hydrolysis of ATP/dATP and the binding of cytochrome c promote 
      APAF-1 oligomerization, forming a large multimeric APAF-1.cytochrome c complex.
      Such a complex can be isolated using gel filtration chromatography and is by
      itself sufficient to recruit and activate procaspase-9. The stoichiometric ratio 
      of procaspase-9 to APAF-1 is approximately 1 to 1 in the complex. Once activated,
      caspase-9 disassociates from the complex and becomes available to cleave and
      activate downstream caspases such as caspase-3.
FAU - Zou, H
AU  - Zou H
AD  - Howard Hughes Medical Institute and Department of Biochemistry, University of
      Texas Southwestern Medical Center, Dallas, Texas 75235, USA.
FAU - Li, Y
AU  - Li Y
FAU - Liu, X
AU  - Liu X
FAU - Wang, X
AU  - Wang X
LA  - eng
GR  - GMRO1-57158/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (APAF1 protein, human)
RN  - 0 (Apoptotic Protease-Activating Factor 1)
RN  - 0 (Cytochrome c Group)
RN  - 0 (DNA Primers)
RN  - 0 (Enzyme Precursors)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.4.22.- (CASP9 protein, human)
RN  - EC 3.4.22.- (Caspase 9)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Apoptosis/*physiology
MH  - Apoptotic Protease-Activating Factor 1
MH  - Base Sequence
MH  - Caspase 9
MH  - Caspases/*metabolism
MH  - Cytochrome c Group/*physiology
MH  - DNA Primers
MH  - Enzyme Activation
MH  - Enzyme Precursors/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Hydrolysis
MH  - Molecular Sequence Data
MH  - Organelles/*physiology
MH  - Protein Binding
MH  - Proteins/*physiology
MH  - Recombinant Proteins/metabolism
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - 10.1074/jbc.274.17.11549 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 23;274(17):11549-56. doi: 10.1074/jbc.274.17.11549.