PMID- 10206952
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 17
DP  - 1999 Apr 23
TI  - Molecular cloning of a novel alpha2,3-sialyltransferase (ST3Gal VI) that
      sialylates type II lactosamine structures on glycoproteins and glycolipids.
PG  - 11479-86
AB  - A novel member of the human CMP-NeuAc:beta-galactoside alpha2,
      3-sialyltransferase (ST) subfamily, designated ST3Gal VI, was identified based on
      BLAST analysis of expressed sequence tags, and a cDNA clone was isolated from a
      human melanoma line library. The sequence of ST3Gal VI encoded a type II membrane
      protein with 2 amino acids of cytoplasmic domain, 32 amino acids of transmembrane
      region, and a large catalytic domain with 297 amino acids; and showed homology to
      previously cloned ST3Gal III, ST3Gal IV, and ST3Gal V at 34, 38, and 33%,
      respectively. Extracts from L cells transfected with ST3Gal VI cDNA in a
      expression vector and a fusion protein with protein A showed an enzyme activity
      of alpha2, 3-sialyltransferase toward Galbeta1,4GlcNAc structure on glycoproteins
      and glycolipids. In contrast to ST3Gal III and ST3Gal IV, this enzyme exhibited
      restricted substrate specificity, i.e. it utilized Galbeta1,4GlcNAc on
      glycoproteins, and neolactotetraosylceramide and neolactohexaosylceramide, but
      not lactotetraosylceramide, lactosylceramide, or asialo-GM1. Consequently, these 
      data indicated that this enzyme is involved in the synthesis of
      sialyl-paragloboside, a precursor of sialyl-Lewis X determinant.
FAU - Okajima, T
AU  - Okajima T
AD  - Department of Biochemistry, Nagoya University School of Medicine, Tsurumai,
      Nagoya 466-0065, Japan.
FAU - Fukumoto, S
AU  - Fukumoto S
FAU - Miyazaki, H
AU  - Miyazaki H
FAU - Ishida, H
AU  - Ishida H
FAU - Kiso, M
AU  - Kiso M
FAU - Furukawa, K
AU  - Furukawa K
FAU - Urano, T
AU  - Urano T
FAU - Furukawa, K
AU  - Furukawa K
LA  - eng
SI  - GENBANK/AB022918
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Amino Sugars)
RN  - 0 (DNA, Complementary)
RN  - 0 (Glycolipids)
RN  - 0 (Glycoproteins)
RN  - 0 (Sialic Acids)
RN  - 13000-25-4 (lactosamine)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - EC 2.4.99.4 (beta-galactoside alpha-2,3-sialyltransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Sugars/*metabolism
MH  - Base Sequence
MH  - Chromatography, Thin Layer
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Glycolipids/chemistry/*metabolism
MH  - Glycoproteins/chemistry/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
MH  - Sialic Acids/*metabolism
MH  - Sialyltransferases/*genetics/metabolism
MH  - Substrate Specificity
MH  - Tumor Cells, Cultured
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - 10.1074/jbc.274.17.11479 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 23;274(17):11479-86. doi: 10.1074/jbc.274.17.11479.