PMID- 10206335
OWN - NLM
STAT- MEDLINE
DCOM- 19990610
LR  - 20190717
IS  - 0003-9969 (Print)
IS  - 0003-9969 (Linking)
VI  - 44
IP  - 2
DP  - 1999 Feb
TI  - The immunohistochemical localization of the interferon-gamma and granulocyte
      colony-stimulating factor receptors during early amelogenesis in rat molars.
PG  - 173-81
AB  - Previous studies, in which the known janus kinase and signal transducer and
      activator of transcription (STAT) isoforms were immunohistochemically mapped in
      developing rat molars, implicated a sizeable list of cytokine superfamily
      receptor (CSR)/signal-transduction pathway (STP) linkages in the cells of the
      enamel organ involved in the events leading directly to early amelogenesis.
      Various combinations of upregulated janus kinases and STATs are known to be
      linked to single or small groups of CSRs. On the basis of the previous
      observations it was hypothesized that the interferon-gamma receptor (IFNgamma r) 
      and the granulocyte colony-stimulating factor receptor (G-CSF receptor) would be 
      localized in specific sites in the cells of the enamel organ during early
      amelogenesis. To verify this, whole-head, freeze-dried sections were here
      obtained at the level of the mandibular first and second molar from newborn and
      5-day-old rats. These sections were not demineralized or fixed, reducing the
      possibility of false-negative results. Antibodies to the IFNgamma r and the G-CSF
      receptor were localized using a modification of the avidin-biotin complex method.
      In the newborn rats, IFNgamma r was localized in the preameloblasts in the
      cervical loop, the proximal and distal ends of presecretory ameloblasts, the
      outer enamel epithelium, the dental lamina, and in bone. In 5-day-old rats, it
      was confined to the proximal ends of the presecretory and secretory ameloblasts. 
      The G-CSF receptor was observed in the molars of newborn rats in the
      preameloblasts, the proximal and distal ends of the presecretory ameloblasts,
      outer enamel epithelium, and in bone. In 5-day-old rats, G-CSF receptor was
      localized in the preameloblasts, the proximal ends of presecretory and secretory 
      ameloblasts, the stellate reticulum, the outer enamel epithelium, and in bone.
      These findings indicate that the IFNgamma r and the G-CSF receptor, and their
      downstream STP linkages, are upregulated in the cells of the enamel organ and may
      be involved in the events leading directly to early enamel formation.
FAU - Otsuji, W
AU  - Otsuji W
AD  - The Department of Pediatric Dentistry, Asahi University School of Dentistry,
      Motosu, Gifu, Japan.
FAU - Tanase, S
AU  - Tanase S
FAU - Yoshida, S
AU  - Yoshida S
FAU - Bawden, J W
AU  - Bawden JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Receptors, Granulocyte Colony-Stimulating Factor)
RN  - 0 (Receptors, Interferon)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (Trans-Activators)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Jak1 protein, rat)
RN  - EC 2.7.10.2 (Janus Kinase 1)
SB  - D
SB  - IM
MH  - Age Factors
MH  - Alveolar Process/cytology/metabolism
MH  - Ameloblasts/metabolism
MH  - Amelogenesis/*physiology
MH  - Animals
MH  - Animals, Newborn
MH  - DNA-Binding Proteins/analysis
MH  - Dental Enamel/*chemistry
MH  - Enamel Organ/cytology/metabolism
MH  - Epithelial Cells/metabolism
MH  - Granulocyte Colony-Stimulating Factor/*analysis
MH  - Immunohistochemistry
MH  - Interferon-gamma/*analysis
MH  - Janus Kinase 1
MH  - Molar
MH  - Protein Isoforms/analysis
MH  - Protein-Tyrosine Kinases/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cytokine/analysis
MH  - Receptors, Granulocyte Colony-Stimulating Factor/*analysis
MH  - Receptors, Interferon/*analysis
MH  - STAT1 Transcription Factor
MH  - Signal Transduction
MH  - Trans-Activators/analysis
MH  - Up-Regulation
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - S0003996998000922 [pii]
AID - 10.1016/s0003-9969(98)00092-2 [doi]
PST - ppublish
SO  - Arch Oral Biol. 1999 Feb;44(2):173-81. doi: 10.1016/s0003-9969(98)00092-2.